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- 03:34, 26 April 2010 S8600021 talk contribs uploaded File:Mouse- Hand2 forelimb buds.jpg (Early deletion of Hand2 in mouse forelimb buds phenocopies the Shh loss-of-function skeletal phenotype. (A) Whole mount in situ hybridization detects Hand2 transcripts in wild-type (Wt) and mouse embryos that lack the Hand2 gene in their forelimb bud mes)