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- 16:38, 22 March 2010 S8600021 talk contribs uploaded File:Canonical Wnt signaling in Wnt5a KO limb buds.jpg (Canonical Wnt signaling was increased in the Wnt-5a-/- limb buds. Gene expression in embryos was examined by whole mount in situ hybridization. (A) At 12.5 dpc, the expression of Sox9, which marks early chondrogenesis, was not detected in the distal-m)