File:Mouse CT E10.5 head.jpg

Mouse Embryo Computed Tomography

Identically scaled renderings representing 3D μCT scans of embryos (E10.5 head according to paper methods) subjected to different fixation and scanning procedures.

A - 4% formaldehyde and secondary Bouin's fixation.

B - 4% formaldehyde + 1% glutaraldehyde with Iothalamate meglumine used as a contrast agent.

C - 4% formaldehyde + 5% glutaraldehyde plus contrast agent.

All embryos are shown in right 3/4 inferolateral view.

"Typical μCT renderings are represented by the embryos depicted in Figure 2A-C. The combination of aldehyde fixation and Cysto Conray® yields images (Figure 2B-C) with a surface quality comparable to embryos fixed with Bouin's solution (Figure 2A). Bouin's-fixed embryos appear have a rougher surface texture compared to those fixed with either glutaraldehyde mixture (Figure 2B-C). The identically scaled images of Bouin's fixed embryos appeared generally smaller compared to those fixed with glutaraldehyde mixtures, but a comparison of μCT images only cannot indicate the relative roles of fixation-induced tissue shrinkage or swelling."

Original File name: 1471-213X-10-18-2-l.jpg http://www.biomedcentral.com/1471-213X/10/18/figure/F2

Reference

<pubmed>20163731</pubmed>| BMC Developmental Biology

© 2010 Schmidt et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.