File:Human cytomegalovirus beta-catenin juxtanuclear region.jpg
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Human Cytomegalovirus infection inhibits β-catenin nuclear translocation in EVTs
HCMV infection leads to β-catenin protein accumulation in a discrete juxtanuclear region.
- Extravillous trophoblast (EVT) cell line (SGHPL-4) derived from first trimester placenta.
- Cells were seeded on glass coverslips and infected with Towne-GFP (MOI of 1–2) or mock-infected for 48 hr.
- Cells were then treated with 150 ng/ml Wnt-3A or 20 mM LiCl for 6 hr followed by immunostaining for β-catenin using a mouse anti-β catenin antibody, followed by a goat anti-mouse secondary IgG conjugated to AlexaFluor 555.
- Nuclei were counterstained with DAPI. Mouse IgG was used as an isotype control. GFP positive cells represent infected cells. Arrowheads point to nuclear accumulation of β-catenin. Arrows indicate aggregation of β-catenin in infected cells.
Reference
<pubmed>23071438</pubmed>| PLoS Pathog.
HCMV panels cropped from Figure 5. HCMV infection inhibits β-catenin nuclear translocation in EVTs. Journal.ppat.1002959.g005.jpg
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| current | 01:44, 6 November 2012 | | 908 × 1,000 (132 KB) | Z8600021 (talk | contribs) | ==Human Cytomegalovirus infection inhibits β-catenin nuclear translocation in EVTs== HCMV infection leads to β-catenin protein accumulation in a discrete juxtanuclear region. * Extravillous trophoblast (EVT) cell line (SGHPL-4) derived from first tri |
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