User talk:Z3251292: Difference between revisions

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*Process of IVF
*Process of IVF
::* Ovarian hyperstimulation
::* Ovarian hyperstimulation
*** two phase during menstrual cycle: lacteal phase and follicular phase
::** two phase during menstrual cycle: lacteal phase and follicular phase
*** about 30 Follicles grows to large size in every mentrual cycle, but only one survived to the ovulation.
::**about 30 Follicles grows to large size in every mentrual cycle, but only one survived to the ovulation.
*** FSH drove the growth of follicles, also control the growth of follicles (pituitary down regulation), used to stimulate the mature of multiple follicles (10-30).
::** FSH drove the growth of follicles, also control the growth of follicles (pituitary down regulation), used to stimulate the mature of multiple follicles (10-30).
*** Estrogen....
::** Estrogen....
*** Monitoring by transvirgena ultrasound.
::** Monitoring by transvirgena ultrasound.
*** LH containing hormones injected to women to stimulate ovulation.
*** LH containing hormones injected to women to stimulate ovulation.


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::* Sperm retrieval via ejaculation or surgery
::* Sperm retrieval via ejaculation or surgery
::* In Vitro Fertilisation
::* In Vitro Fertilisation
*** intra-cytoplasmic sperm injection (ICSI)
::** intra-cytoplasmic sperm injection (ICSI)
** Embryo culture
** Embryo culture
*** day1- form Zygote
::** day1- form Zygote
*** day3- divide to 8 blastomeres
::** day3- divide to 8 blastomeres
*** day5- frozen, and transfer to uterus
::** day5- frozen, and transfer to uterus
::* Transfer of the embryo(s)
::* Transfer of the embryo(s)



Revision as of 13:23, 14 August 2015

Talk

  • Timeline of The artificial reproductive technology revolution
  • 1984 donor egg pregnancies
  • 1984 frozen embryos
  • 1960 Ovulation induction
  • 1960 Donor sperm
  • 1978 IVF
  • 1988 Pre-implantation genetic diagnosis
  • 1992 Sperm injection
  • 1994 Blastocyst development
  • 1998 Reliable sperm sex selection
  • 2000 Stem cells and cloning and gene transfer
  • 2005 Ovarian reserve testing
  • 2008 Oocyte freezing, social freezing
  • 2013 Whole genome screening of embryos
  • Process of IVF
  • Ovarian hyperstimulation
    • two phase during menstrual cycle: lacteal phase and follicular phase
    • about 30 Follicles grows to large size in every mentrual cycle, but only one survived to the ovulation.
    • FSH drove the growth of follicles, also control the growth of follicles (pituitary down regulation), used to stimulate the mature of multiple follicles (10-30).
    • Estrogen....
    • Monitoring by transvirgena ultrasound.
      • LH containing hormones injected to women to stimulate ovulation.
  • Ooocyte Pick-Up(OPU)
  • Sperm retrieval via ejaculation or surgery
  • In Vitro Fertilisation
    • intra-cytoplasmic sperm injection (ICSI)
    • Embryo culture
    • day1- form Zygote
    • day3- divide to 8 blastomeres
    • day5- frozen, and transfer to uterus
  • Transfer of the embryo(s)
  • New technologies
    • Preimplantation genetic diagnosis(PGD) of embryo. eg, fibrosis-premature death
      • Embryo Biopsy for PGD
      • analysis of blastomeres for PGD
        • FISH
        • PCR
      • What to be detected in an embryo
        • Aneuploidy
        • trisomy 21
        • translocations
        • single gene defects(eg cystic fibrosis)
    • Ovarian reserve and the natural cycle
      • blood test measure hormones(AMH, AFC)
      • Egg banking ('social freezing')
    • Fertility preservation for cancer
    • Oocyte in vitro maturation (IVM)
      • putting oocytes in vitro and culture 24 hours before IVF (Complex medium , protein, FSH, EGF, LH, E2 cysteamine)
      • comparing to conventional IVF, no Hormones required to give to mother for the maturation of oocyte.