User talk:Z3251292: Difference between revisions
From Embryology
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===Talk=== | ===Talk=== | ||
*Timeline of The artificial reproductive technology revolution | |||
::*1984 donor egg pregnancies | ::*1984 donor egg pregnancies | ||
::*1984 frozen embryos | |||
::*1960 Ovulation induction | |||
::*1960 Donor sperm | |||
::*1978 IVF | |||
::*1988 Pre-implantation genetic diagnosis | |||
::*1992 Sperm injection | |||
::*1994 Blastocyst development | |||
::*1998 Reliable sperm sex selection | |||
::*2000 Stem cells and cloning and gene transfer | |||
::*2005 Ovarian reserve testing | |||
::*2008 Oocyte freezing, social freezing | |||
::*2013 Whole genome screening of embryos | |||
*Process of IVF | |||
::* Ovarian hyperstimulation | |||
*** two phase during menstrual cycle: lacteal phase and follicular phase | *** two phase during menstrual cycle: lacteal phase and follicular phase | ||
*** about 30 Follicles grows to large size in every mentrual cycle, but only one survived to the ovulation. | *** about 30 Follicles grows to large size in every mentrual cycle, but only one survived to the ovulation. | ||
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*** LH containing hormones injected to women to stimulate ovulation. | *** LH containing hormones injected to women to stimulate ovulation. | ||
::* Ooocyte Pick-Up(OPU) | |||
::* Sperm retrieval via ejaculation or surgery | |||
::* In Vitro Fertilisation | |||
*** intra-cytoplasmic sperm injection (ICSI) | *** intra-cytoplasmic sperm injection (ICSI) | ||
** Embryo culture | ** Embryo culture | ||
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*** day3- divide to 8 blastomeres | *** day3- divide to 8 blastomeres | ||
*** day5- frozen, and transfer to uterus | *** day5- frozen, and transfer to uterus | ||
::* Transfer of the embryo(s) | |||
* New technologies | |||
** Preimplantation genetic diagnosis(PGD) of embryo. eg, fibrosis-premature death | |||
*** Embryo Biopsy for PGD | *** Embryo Biopsy for PGD | ||
*** analysis of blastomeres for PGD | *** analysis of blastomeres for PGD | ||
| Line 48: | Line 48: | ||
****translocations | ****translocations | ||
****single gene defects(eg cystic fibrosis) | ****single gene defects(eg cystic fibrosis) | ||
** Ovarian reserve and the natural cycle | |||
***blood test measure hormones(AMH, AFC) | ***blood test measure hormones(AMH, AFC) | ||
***Egg banking ('social freezing') | ***Egg banking ('social freezing') | ||
** Fertility preservation for cancer | |||
** Oocyte in vitro maturation (IVM) | |||
*** putting oocytes in vitro and culture 24 hours before IVF (Complex medium , protein, FSH, EGF, LH, E2 cysteamine) | *** putting oocytes in vitro and culture 24 hours before IVF (Complex medium , protein, FSH, EGF, LH, E2 cysteamine) | ||
*** comparing to conventional IVF, no Hormones required to give to mother for the maturation of oocyte. | *** comparing to conventional IVF, no Hormones required to give to mother for the maturation of oocyte. | ||
Revision as of 13:22, 14 August 2015
Talk
- Timeline of The artificial reproductive technology revolution
- 1984 donor egg pregnancies
- 1984 frozen embryos
- 1960 Ovulation induction
- 1960 Donor sperm
- 1978 IVF
- 1988 Pre-implantation genetic diagnosis
- 1992 Sperm injection
- 1994 Blastocyst development
- 1998 Reliable sperm sex selection
- 2000 Stem cells and cloning and gene transfer
- 2005 Ovarian reserve testing
- 2008 Oocyte freezing, social freezing
- 2013 Whole genome screening of embryos
- Process of IVF
- Ovarian hyperstimulation
- two phase during menstrual cycle: lacteal phase and follicular phase
- about 30 Follicles grows to large size in every mentrual cycle, but only one survived to the ovulation.
- FSH drove the growth of follicles, also control the growth of follicles (pituitary down regulation), used to stimulate the mature of multiple follicles (10-30).
- Estrogen....
- Monitoring by transvirgena ultrasound.
- LH containing hormones injected to women to stimulate ovulation.
- Ooocyte Pick-Up(OPU)
- Sperm retrieval via ejaculation or surgery
- In Vitro Fertilisation
- intra-cytoplasmic sperm injection (ICSI)
- Embryo culture
- day1- form Zygote
- day3- divide to 8 blastomeres
- day5- frozen, and transfer to uterus
- Transfer of the embryo(s)
- New technologies
- Preimplantation genetic diagnosis(PGD) of embryo. eg, fibrosis-premature death
- Embryo Biopsy for PGD
- analysis of blastomeres for PGD
- FISH
- PCR
- What to be detected in an embryo
- Aneuploidy
- trisomy 21
- translocations
- single gene defects(eg cystic fibrosis)
- Ovarian reserve and the natural cycle
- blood test measure hormones(AMH, AFC)
- Egg banking ('social freezing')
- Fertility preservation for cancer
- Oocyte in vitro maturation (IVM)
- putting oocytes in vitro and culture 24 hours before IVF (Complex medium , protein, FSH, EGF, LH, E2 cysteamine)
- comparing to conventional IVF, no Hormones required to give to mother for the maturation of oocyte.
- Preimplantation genetic diagnosis(PGD) of embryo. eg, fibrosis-premature death
