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==Abnormal cytokinesis and apoptosis in UBE3A knockdown cells== | |||
(A) Note cells undergoing cytokinesis with elongated nuclear morphology giving rise to abnormal number of nuclei. An extended midbody (arrowheads) and micronuclei (arrows) can be seen in all the panels. ASPM was found to be diffusely present at the midbody. Scale=2 µm. (B) Quantitation of apoptosis by in vivo detection of caspase-3 activity. Note UBE3A shRNA clones showed a significant increase in apoptotic cells as compared to scrambled clones. Cells were analyzed by flow cytometry using FL-1 channel (10,000 cells were measured for each sample). Mean ± SEM values for the samples is as follows: scrambled clone P=6.150±0.2972, scrambled clone K=4.920±0.0986, UBE3AshRNA clone T=7.700±0.2663, and UBE3AshRNA clone U=14.99±0.2929. Data are representative of three independent experiments. Unpaired Student's t-test was used to determine the significance of difference between scrambled and UBE3A knockdown clones. | (A) Note cells undergoing cytokinesis with elongated nuclear morphology giving rise to abnormal number of nuclei. An extended midbody (arrowheads) and micronuclei (arrows) can be seen in all the panels. ASPM was found to be diffusely present at the midbody. Scale=2 µm. (B) Quantitation of apoptosis by in vivo detection of caspase-3 activity. Note UBE3A shRNA clones showed a significant increase in apoptotic cells as compared to scrambled clones. Cells were analyzed by flow cytometry using FL-1 channel (10,000 cells were measured for each sample). Mean ± SEM values for the samples is as follows: scrambled clone P=6.150±0.2972, scrambled clone K=4.920±0.0986, UBE3AshRNA clone T=7.700±0.2663, and UBE3AshRNA clone U=14.99±0.2929. Data are representative of three independent experiments. Unpaired Student's t-test was used to determine the significance of difference between scrambled and UBE3A knockdown clones. | ||
===Reference=== | |||
<pubmed>21633703 </pubmed> | |||
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102111/?tool=pubmed | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102111/?tool=pubmed | ||
Copyright Singhmar, Kumar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | 110811-abnormal_cytokinesis_and_apop._image.jpg | ||
====Copyright==== | |||
Singhmar, Kumar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||
--[[User:Z8600021|Mark Hill]] ([[User talk:Z8600021|talk]]) 20:03, 2 December 2013 (EST) Information formatted and referenced correctly. | |||
[[Category:Mitosis]] [[Category:Apoptosis]] | |||
Latest revision as of 19:03, 2 December 2013
Abnormal cytokinesis and apoptosis in UBE3A knockdown cells
(A) Note cells undergoing cytokinesis with elongated nuclear morphology giving rise to abnormal number of nuclei. An extended midbody (arrowheads) and micronuclei (arrows) can be seen in all the panels. ASPM was found to be diffusely present at the midbody. Scale=2 µm. (B) Quantitation of apoptosis by in vivo detection of caspase-3 activity. Note UBE3A shRNA clones showed a significant increase in apoptotic cells as compared to scrambled clones. Cells were analyzed by flow cytometry using FL-1 channel (10,000 cells were measured for each sample). Mean ± SEM values for the samples is as follows: scrambled clone P=6.150±0.2972, scrambled clone K=4.920±0.0986, UBE3AshRNA clone T=7.700±0.2663, and UBE3AshRNA clone U=14.99±0.2929. Data are representative of three independent experiments. Unpaired Student's t-test was used to determine the significance of difference between scrambled and UBE3A knockdown clones.
Reference
<pubmed>21633703 </pubmed>
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102111/?tool=pubmed
110811-abnormal_cytokinesis_and_apop._image.jpg
Copyright
Singhmar, Kumar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
--Mark Hill (talk) 20:03, 2 December 2013 (EST) Information formatted and referenced correctly.
File history
Yi efo/eka'e gwa ebo wo le nyangagi wuncin ye kamina wunga tinya nan
| Gwalagizhi | Nyangagi | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 14:06, 11 August 2011 |  | 430 × 647 (138 KB) | Z3291643 (talk | contribs) | 110811-abnormal_cytokinesis_and_apop._image.jpg (A) Note cells undergoing cytokinesis with elongated nuclear morphology giving rise to abnormal number of nuclei. An extended midbody (arrowheads) and micronuclei (arrows) can be seen in all the panels. ASP |
| 13:57, 11 August 2011 |  | 430 × 647 (138 KB) | Z3291643 (talk | contribs) | 110811-abnormal_cytokinesis_and_apop._image.jpg Abnormal cytokinesis and apoptosis in UBE3A knockdown cells. (A) Note cells undergoing cytokinesis with elongated nuclear morphology giving rise to abnormal number of nuclei. An extended midbody (arrowheads |
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