File:Rat kidney HIM 04.jpg: Difference between revisions

Latest revision as of 15:12, 14 May 2014

Adult Rat Kidney Collecting Duct Principal Cell and Intercalated Cell HIM

PC - Principal cell has one long, solitary cilium and numerous short, stubby microvilli.
IC - Intercalated cell has numerous elaborate apical microplicae, ridge-like folds of the plasmalemma, and no cilium.

Bar = 2 µm.

Collecting duct principal cells mediate the collecting duct's influence on sodium and potassium balance through sodium and potassium channels located on the cell's apical membrane. Intercalated cells come in α and β forms and have a role in acid-base homeostasis.

A new technique called Helium Ion Microscopy (HIM) gives similar high resolution 3D images, using a scanning beam of He⁺ ions, and fixed tissue without the coating required for SEM.

Reference

<pubmed>23505418</pubmed>| PMC3591388 | PLoS One.

Copyright

© 2013 Rice et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Journal.pone.0057051.g006 Original figure 6 Panel A cropped from full image, adjusted in size, contrast and labelling. Journal.pone.0057051.g006a.jpg

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Yi efo/eka'e gwa ebo wo le nyangagi wuncin ye kamina wunga tinya nan

	Gwalagizhi	Nyangagi	Dimensions	User	Comment
current	15:05, 14 May 2014		599 × 602 (142 KB)	Z8600021 (talk \| contribs)	==Adult Rat Kidney Collecting Duct Principal Cell HIM== Each principal cell has one long, solitary cilium and numerous short, stubby microvilli. Collecting duct principal cells mediate the collecting duct's influence on sodium and potassium balance thr...

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Scanning Electron Microscopy

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Copyright holder	Creative Commons Attribution License
Copyright status	Copyright status not set
Software used	Adobe Photoshop CS2 Windows
Short title	Figure 6
IIM version	2

@@ Line 1: / Line 1: @@
-==Adult Rat Kidney Collecting Duct Principal Cell HIM==
+==Adult Rat Kidney Collecting Duct Principal Cell and Intercalated Cell HIM==
-Each principal cell has one long, solitary cilium and numerous short, stubby microvilli. Collecting duct principal cells mediate the collecting duct's influence on sodium and potassium balance through sodium and potassium channels located on the cell's apical membrane.
-High magnification view of a principal cell cilium (Bar = 200 nm). At its base, a concentric pattern of surface protrusions (arrows) can be seen in the position of the ciliary necklace. A similar structure is shown on another principal cell cilium in the inset (arrows, Bar = 100 nm).
+* '''PC''' - Principal cell has one long, solitary cilium and numerous short, stubby microvilli.
+* '''IC''' - Intercalated cell  has numerous elaborate apical microplicae, ridge-like folds of the plasmalemma, and no cilium.
+Bar = 2 µm.
+Collecting duct principal cells mediate the collecting duct's influence on sodium and potassium balance through sodium and potassium channels located on the cell's apical membrane. Intercalated cells come in α and β forms and have a role in acid-base homeostasis.
 A new technique called Helium Ion Microscopy (HIM) gives similar high resolution 3D images, using a scanning beam of He<sup>+</sup> ions, and fixed tissue without the coating required for SEM.
-Bar = 500 nm
 {{Rat kidney HIM links}}