Template talk:Cahalan: Difference between revisions

Latest revision as of 17:42, 3 March 2013

Lymphocyte Migration Speeds

T cells - 10–12 μm/min in the follicle diffuse cortex, peak velocities up to 30 μm/min.

B cells - 6 μm/min in the follicle diffuse cortex, peak velocities up to 20 μm/min.

Both cortical T cells and follicular B cells move in random directions following "guide cells".

Lymphocyte Guide Cells

FDC - Follicular Dendritic Cells, may guide B cells in the follicle.

FRC - Fibroblastic Reticular Cells, may guide T cells in the follicle.

Lymph Node 2-Photon labelling

Red - Alexa Fluor 633–conjugated WGA image channel
- sinus space both as a weakly stained area in the WGA image channel

Green - T lymphocytes labeled with 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester.

Green - B lymphocytes labelled with antibody to B220 (fixed tissue)

Original template

Links: Immune System Development | Lymph Node Development | Quicktime | Flash

Movie Source: Michael D. Cahalan, Professor and Chair, Department of Physiology and Biophysics, University of California, Irvine. Lab Two-photon imaging movies reproduced with permission of Prof. Cahalan. Movies show lymphocyte traffic through adult lymph nodes. Cells were isolated from spleens and lymph nodes of BALB/c mice.

References: <pubmed>16273098</pubmed>| Immunology <pubmed>18173372</pubmed>

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	:'''Movie Source:''' Michael D. Cahalan, Professor and Chair, Department of Physiology and Biophysics, University of California, Irvine. [http://crt.biomol.uci.edu Lab] Two-photon imaging movies reproduced with permission of Prof. Cahalan. Movies show lymphocyte traffic through adult lymph nodes. Cells were isolated from spleens and lymph nodes of BALB/c mice.		:'''Movie Source:''' Michael D. Cahalan, Professor and Chair, Department of Physiology and Biophysics, University of California, Irvine. [http://crt.biomol.uci.edu Lab] Two-photon imaging movies reproduced with permission of Prof. Cahalan. Movies show lymphocyte traffic through adult lymph nodes. Cells were isolated from spleens and lymph nodes of BALB/c mice.

	:'''T Cell MP4:''' [[Quicktime_Movie_-_Mouse_Lymph_Node_1\|Transendothelial migration]] \| [[Quicktime_Movie_-_Mouse_Lymph_Node_2\|T cell zone]] \| [[Quicktime_Movie_-_Mouse_Lymph_Node_3\|Medullary sinus]] \| [[Quicktime_Movie_-_Mouse_Lymph_Node_4\|Sinus endothelial barrier]] \| [[Quicktime_Movie_-_Mouse_Lymph_Node_5\|Bi-directional traffic]] \| [[Quicktime_Movie_-_Mouse_Lymph_Node_6\|cross the sinus endothelial barrier]]