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Human Spermatozoa Phospholipase C zeta Localization

Developmental localization of PLCζ in human (B, C) testes utilizing anti-hmPLCζ antibody. In human, there are six stages (I–VI) of the cycle of the seminiferous epithelium.

B - (stage II) PLCζ accumulates over the acrosomic vesicle (AV) of step 2 spermatids. Little immunoreactivity is found elsewhere in the epithelium and the elongated spermatids (arrowheads) appear unreactive.
C - (stage IV) step 4 spermatids the fully formed acrosome is intensely labeled (arrows).

Bars = 20 µm

Reference

<pubmed>22428063</pubmed>| PLoS One.

Citation: Aarabi M, Yu Y, Xu W, Tse MY, Pang SC, et al. (2012) The Testicular and Epididymal Expression Profile of PLCζ in Mouse and Human Does Not Support Its Role as a Sperm-Borne Oocyte Activating Factor. PLoS ONE 7(3): e33496. doi:10.1371/journal.pone.0033496

Copyright: © 2012 Aarabi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Journal.pone.0033496.g002.jpg

Panel B and C from Figure 2. Developmental localization of PLCζ in mouse (A) and human (B, C) testes utilizing anti-hmPLCζ antibody.

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current	12:55, 6 May 2012		1,000 × 571 (119 KB)	Z8600021 (talk \| contribs)	Human Spermatozoa Phospholipase C zeta Localization== Figure 2. Developmental localization of PLCζ in mouse (A) and human (B, C) testes utilizing anti-hmPLCζ antibody. Similar results were obtained with anti-EF antibody in mouse and human and anti-hPLC

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Spermatozoa Development

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 ==Human Spermatozoa Phospholipase C zeta Localization==
-Figure 2. Developmental localization of PLCζ in mouse (A) and human (B, C) testes utilizing anti-hmPLCζ antibody.
+Developmental localization of PLCζ in human (B, C) testes utilizing anti-hmPLCζ antibody. In human, there are six stages (I–VI) of the cycle of the seminiferous epithelium.
-Similar results were obtained with anti-EF antibody in mouse and human and anti-hPLCζ in human sections. (A) Immunostaining originates at the beginning of acrosome formation. In stage III it appears confined to the acrosomic vesicle (AV) of step 3 spermatids as seen in detail in the inset; note that acrosomic granule is most immunoreactive. In stage VII immunostaining is intense within the acrosome, capping a portion of the nucleus (arrowheads) of the step 7 round spermatids as seen in more detail in the inset. In stages XI and XII immunostaining resides in the head of elongating spermatids in steps 11 and 12, respectively (arrows). However, by step 14 (stages II and III) the intensity of immunostaining in elongated spermatid heads has diminished significantly (arrows). (B, C) In human, there are six stages (I–VI) of the cycle of the seminiferous epithelium. As seen in B, PLCζ accumulates over the acrosomic vesicle (AV) of step 2 spermatids in stage II. Little immunoreactivity is found elsewhere in the epithelium and the elongated spermatids (arrowheads) appear unreactive. In step 4 spermatids (stage IV, see C), the fully formed acrosome is intensely labeled (arrows). Bars = 20 µm; Bars in insets = 5 µm.
+* '''B''' - (stage II) PLCζ accumulates over the acrosomic vesicle (AV) of step 2 spermatids. Little immunoreactivity is found elsewhere in the epithelium and the elongated spermatids (arrowheads) appear unreactive.
+* '''C''' - (stage IV) step 4 spermatids the fully formed acrosome is intensely labeled (arrows).
-http://www.ncbi.nlm.nih.gov/pubmed/22428063
+Bars = 20 µm
+===Reference===
+<pubmed>22428063</pubmed>| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0033496 PLoS One.]
-http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0033496
 Citation: Aarabi M, Yu Y, Xu W, Tse MY, Pang SC, et al. (2012) The Testicular and Epididymal Expression Profile of PLCζ in Mouse and Human Does Not Support Its Role as a Sperm-Borne Oocyte Activating Factor. PLoS ONE 7(3): e33496. doi:10.1371/journal.pone.0033496