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'''FRC''' - Fibroblastic Reticular Cells, may guide T cells in the follicle. | '''FRC''' - Fibroblastic Reticular Cells, may guide T cells in the follicle. | ||
2-Photon labelling | |||
* Red - Alexa Fluor 633–conjugated WGA image channel | |||
** sinus space both as a weakly stained area in the WGA image channel | |||
* Green - T lymphocytes labeled with 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester. | |||
* Green - B lymphocytes labelled with antibody to B220 (fixed tissue) | |||
Revision as of 13:38, 22 February 2011
Lymphocyte Migration Speeds
T cells - 10–12 μm/min in the follicle diffuse cortex, peak velocities up to 30 μm/min.
B cells - 6 μm/min in the follicle diffuse cortex, peak velocities up to 20 μm/min.
Both cortical T cells and follicular B cells move in random directions following "guide cells".
Lymphocyte Guide Cells
FDC - Follicular Dendritic Cells, may guide B cells in the follicle.
FRC - Fibroblastic Reticular Cells, may guide T cells in the follicle.
2-Photon labelling
- Red - Alexa Fluor 633–conjugated WGA image channel
- sinus space both as a weakly stained area in the WGA image channel
- Green - T lymphocytes labeled with 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester.
- Green - B lymphocytes labelled with antibody to B220 (fixed tissue)
