Movie - Early Division Chromosomes: Difference between revisions

Revision as of 14:28, 24 November 2010

<Flowplayer height="400" width="380" autoplay="true">Mouse_zygote_division_02.flv</Flowplayer>

Movie shows mitotic division of the early mouse embryo starting at the zygote stage.

The elapsed time from the start of acquisition (days:hours:minutes) is indicated.

Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively).

DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin.

Links: Quicktime version | Flash version

Mouse embryo was injected with H2B-mRFP mRNA and Fab313-488. Images of 51 focal planes (2-µm intervals) were captured at 10-min intervals using an inverted microscope (IX-71) with an UPlan-Apochromat 40× NA 1.0 oil immersion objective lens. Maximum projections of H2B-mRFP (top left) and Fab313-488 (top right) are shown with brightfield (BF; bottom left) and merged images (bottom right; H2B and Fab311 are shown in cyan and red, respectively).

The elapsed time from the start of acquisition (days:hours:minutes) is indicated. Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19), as it recognizes histone H3S10ph next to di- or trimethylated H3K9.

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Reference

<pubmed>19995936</pubmed>| JCB

Published December 14, 2009 // JCB vol. 187 no. 6 781-790 The Rockefeller University Press, doi: 10.1083/jcb.200904137 Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase

Copyright

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 [[Media:Mouse_zygote_division_02.mov|Quicktime movie]]
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+Movie shows mitotic division of the early mouse embryo starting at the zygote stage.
-Movie shows mitotic division of the early mouse embryo starting at the zygote stage.
 The elapsed time from the start of acquisition (days:hours:minutes) is indicated.