File:Mice cloned from adult keratinocytes.jpg: Difference between revisions
(Mice cloned from adult skin keratinocytes and other cells by NT. (A) Cloned blastocysts from oocytes reconstructed by nuclear transfer (NT) of bulge follicle keratinocyte nuclei into unfertilized mouse oocytes. The α6+CD34+ bulge keratinocytes were iso) |
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Mice cloned from adult skin keratinocytes and other cells by | ==Mice cloned from adult skin keratinocytes and other cells by Nuclear Transfer== | ||
(A) Cloned blastocysts from oocytes reconstructed by nuclear transfer (NT) of bulge follicle keratinocyte nuclei into unfertilized mouse oocytes. The α6+CD34+ bulge keratinocytes were isolated by FACS from skin of adult female K14-Cre/ROSA-floxed-lacZ mice and used directly in NT. | '''(A)''' Cloned blastocysts from oocytes reconstructed by nuclear transfer (NT) of bulge follicle keratinocyte nuclei into unfertilized mouse oocytes. The α6+CD34+ bulge keratinocytes were isolated by FACS from skin of adult female K14-Cre/ROSA-floxed-lacZ mice and used directly in NT. | ||
(B) Three mice cloned from the same donor mouse, an adult female. The pup on the left is from an α6+CD34+ bulge follicle keratinocyte nucleus, and the two pups on the right are from CC nuclei. | '''(B)''' Three mice cloned from the same donor mouse, an adult female. The pup on the left is from an α6+CD34+ bulge follicle keratinocyte nucleus, and the two pups on the right are from CC nuclei. | ||
(C) Three mice cloned from the same donor mouse, an adult male. The pup on the left is from an α6+CD34+ bulge follicle keratinocyte nucleus, and the two pups on the right are from α6+CD34− keratinocyte nuclei. | '''(C)''' Three mice cloned from the same donor mouse, an adult male. The pup on the left is from an α6+CD34+ bulge follicle keratinocyte nucleus, and the two pups on the right are from α6+CD34− keratinocyte nuclei. | ||
'''(D)''' Six-week-old mice cloned by NT from α6+CD34+ bulge follicle keratinocytes isolated from an adult male. | |||
==Reference== | |||
Proc Natl Acad Sci U S A. 2007 February 20; 104(8): 2738–2743. | Proc Natl Acad Sci U S A. 2007 February 20; 104(8): 2738–2743. | ||
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Copyright © 2007 by The National Academy of Sciences of the USA | Copyright © 2007 by The National Academy of Sciences of the USA | ||
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1815251/?tool=pubmed | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1815251/?tool=pubmed | ||
Revision as of 13:17, 2 October 2010
Mice cloned from adult skin keratinocytes and other cells by Nuclear Transfer
(A) Cloned blastocysts from oocytes reconstructed by nuclear transfer (NT) of bulge follicle keratinocyte nuclei into unfertilized mouse oocytes. The α6+CD34+ bulge keratinocytes were isolated by FACS from skin of adult female K14-Cre/ROSA-floxed-lacZ mice and used directly in NT.
(B) Three mice cloned from the same donor mouse, an adult female. The pup on the left is from an α6+CD34+ bulge follicle keratinocyte nucleus, and the two pups on the right are from CC nuclei.
(C) Three mice cloned from the same donor mouse, an adult male. The pup on the left is from an α6+CD34+ bulge follicle keratinocyte nucleus, and the two pups on the right are from α6+CD34− keratinocyte nuclei.
(D) Six-week-old mice cloned by NT from α6+CD34+ bulge follicle keratinocytes isolated from an adult male.
Reference
Proc Natl Acad Sci U S A. 2007 February 20; 104(8): 2738–2743. Published online 2007 February 13. doi: 10.1073/pnas.0611358104.
Copyright © 2007 by The National Academy of Sciences of the USA
Copyright
Proceedings National Academy of Sciences (PNAS) Liberalization of PNAS copyright policy: Noncommercial use freely allowed Note original Author should be contacted for permission to reuse for Educational purposes. See also PNAS Author Rights and Permission FAQs
- Cozzarelli NR, Fulton KR, Sullenberger DM. Liberalization of PNAS copyright policy: noncommercial use freely allowed. Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12399. PMID15314225 "Our guiding principle is that, while PNAS retains copyright, anyone can make noncommercial use of work in PNAS without asking our permission, provided that the original source is cited."
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1815251/?tool=pubmed
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