File:Organoids - inner ear 01.jpg: Difference between revisions

Summary

CHIR-treated aggregates give rise to inner ear organoids harboring mechanosensitive hair cells

(A-B) Co-localization of two hair cell markers Calb2 and Myo7a (A) or Sox2 and Myo7a (B) in cells lining the luminal surface of a vesicle. (C) Atoh1/nGFP+ cells also express Myo7a and Sox2. (D-F) Cells expressing Brn3C, Myo7a, and Atoh1/nGFP exhibit flask-like morphology with a hair bundle on their apical surface characteristic of vestibular hair cells. Some Myo7a+ cells in day 28 samples have faint Atoh1/nGFP expression (arrows), suggesting that these cells are more mature hair cells than cells expressing strong Atoh1/nGFP expression. (G) The hair bundle marker Espin was observed on the apical surface of Calb2+ hair cells. (H) Representative voltage-gated currents and mechanosensitive currents recorded from day 25 Atoh1/nGFP+ cells in response to voltage injections and hair bundle deflections, respectively. (I) A cluster of Brn3A+ neuronal cell bodies were located near Myo7a+ hair cells. (J) A TUJ1+ neural processes extend and contact Myo7a+ hair cells. (K) The ribbon synapse marker CtBP2 was associated with Myo7a+ cells and TUJ1+ processes. Scale bars, 10 μm (A-G, I-K).

Reference

DeJonge RE, Liu XP, Deig CR, Heller S, Koehler KR & Hashino E. (2016). Modulation of Wnt Signaling Enhances Inner Ear Organoid Development in 3D Culture. PLoS ONE , 11, e0162508. PMID: 27607106 DOI.

Copyright

© 2016 DeJonge et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Fig 5. Journal.pone.0162508.g005.jpg

https://doi.org/10.1371/journal.pone.0162508.g005