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(J Cell Biol. 1991 Jun;113(5):1069-80. Motility of bile canaliculi in the living animal: implications for bile flow. Watanabe N1, Tsukada N, Smith CR, Phillips MJ. Author information Abstract Modern fluorescence microscopic techniques were used to image...)
 
 
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J Cell Biol. 1991 Jun;113(5):1069-80.
==Liver Bile Canaliculi and Sinusoids SEM==
Motility of bile canaliculi in the living animal: implications for bile flow.
 
Watanabe N1, Tsukada N, Smith CR, Phillips MJ.
Scanning electron micrographs of a fractured surface of rat livers. (a) Control liver. Note the chicken wire-like anastomosing network of bile canaliculi. Canalicular lumina are uniformly filled with microvilli.
Author information
 
Abstract
'''arrowheads''' - bile canaliculi (channels shown shown as narrow paired lines)
Modern fluorescence microscopic techniques were used to image the bile canalicular system in the intact rat liver, in vivo. By combining the use of sodium fluorescein secretion into bile, with digitally enhanced fluorescence microscopy and time-lapse video, it was possible to capture and record the canalicular motility events that accompany the secretion of bile in life. Active bile canalicular contractions were found predominantly in zone 1 (periportal) hepatocytes of the liver. The contractile movements were repetitive, forceful, and appeared unidirectional moving bile in a direction towards the portal bile ducts. Contractions were not seen in the network of canaliculi on the surface of the liver. Cytochalasin B administration resulted in reduced canalicular motility, progressive dilation of zone 1 canaliculi, and impairment of bile flow. Canalicular dilations invariably involved the branch points of the canalicular network. The findings add substantively to previous in vitro studies using couplets, and suggest that canalicular contractions contribute physiologically to bile flow in the liver.
 
PMID: 2040644 PMCID: PMC2289005
'''S''' - sinusoid
 
:'''Links:'''
===Reference===
 
{{#pmid:2040644}}
 
{{JCB}}
 
Figure 8 panel A cropped, resized and relabelled with PMID.
 
 
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[[Category:Scanning EM]][[Category:Rat]][[Category:Liver]]

Latest revision as of 16:45, 18 Mayıs 2018

Liver Bile Canaliculi and Sinusoids SEM

Scanning electron micrographs of a fractured surface of rat livers. (a) Control liver. Note the chicken wire-like anastomosing network of bile canaliculi. Canalicular lumina are uniformly filled with microvilli.

arrowheads - bile canaliculi (channels shown shown as narrow paired lines)

S - sinusoid

Links:

Reference

Watanabe N, Tsukada N, Smith CR & Phillips MJ. (1991). Motility of bile canaliculi in the living animal: implications for bile flow. J. Cell Biol. , 113, 1069-80. PMID: 2040644

Copyright

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Figure 8 panel A cropped, resized and relabelled with PMID.



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current16:10, 18 Mayıs 2018Thumbnail for version as of 16:10, 18 Mayıs 20182,000 × 1,333 (350 KB)Z8600021 (talk | contribs)J Cell Biol. 1991 Jun;113(5):1069-80. Motility of bile canaliculi in the living animal: implications for bile flow. Watanabe N1, Tsukada N, Smith CR, Phillips MJ. Author information Abstract Modern fluorescence microscopic techniques were used to image...

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