User:Z3439257

Lab 1

ANAT2341 Lab 1 - Online Assessment

PMID 26043223

Paper Review: This article has firstly proved that the proteins (ZP-1, ZP-2 & ZP-3) in mouse zona pellucida form functional amyloid surrounding the oocytes. Firstly, several experiments such as staining and western blot were performed to confirm that the proteins are forming amyloid structure. Then, the amino acid sequence of the proteins from six different taxa were analysed and essential amyloidogenic sites were identified. It has been described that different ZP proteins will aggregate with each other or self-aggregate in different ways, depends on the amyloidogenic sites it possess. Specifically, ZP-N repeats, which is on of the amyloidogenic sites, has been stated to be related to the recognition of sperm. Therefore, the conclusion that ZP proteins exist in the ZP as functional amyloid and may get involved in prevention of polyspermy and cross-species fertilisation has been come up with. Moreover, since the amyloidogenic sites across species seem to be conserved, the amyloid formed are expected to possess similar functions, making this study on mouse model useful on human researches. Furthermore, some proteins exist in other parts of the body also have the conserved amyloidogenic sites, part or all of their functions may be carried out through their amyloid structure formed.

Amyloid used to be associated with several neurodegenerative and prion diseases in mammals. However, it is becoming increasingly obvious that functional amyloid does exist and possesses a physiological function rather than a pathological function. The further research can be focusing on the specificity of the amyloid structure formed and the functions related. Based on the data published, a question about diagram 2A is raised. It is known that buffer was used to incubate with the PAD beads as the negative control. However, a light band can be observed in the negative control, which is not supposed to happen.

Mark Hill 18 August 2016 - You have added the citation correctly and written a good summary of the article. Quite an interesting paper on protein structure of the ZP, amyloid cross-β sheet fibrillar structure is an interesting association. Unfortunately I have had to take marks off the final assessment as you have not added the reference correctly. Add a link to this reference using its PMID using this code <pubmed>XXXXX</pubmed> replacing the Xs with just the PMID number (no text). You have just added the link, here is the reference: <pubmed>26043223</pubmed> I also fixed your formatting that had a space at the beginning (no deduction for this).

Assessment 3/5

Lab 2

ANAT2341 Lab 2 - Online Assessment

Human trophoblasts stained with different markers

Lab 3

ANAT2341 Lab 3 - Online Assessment

Lab 4

ANAT2341 Lab 4 - Online Assessment

Group Project

2016 Group Project 1

Reference

Jung R, Choi JH, Lee HJ, Kim JK, Kim GJ. Effect of Immortalization-Upregulated Protein-2 (IMUP-2) on Cell Death of Trophoblast. Development & Reproduction. 2013;17(2):99-109. doi:10.12717/DR.2013.17.2.099.

Lab Attendance

Z3439257 (talk) 14:35, 5 August 2016 (AEST) Z3439257 (talk) 14:40, 12 August 2016 (AEST) Z3439257 (talk) Z3439257 (talk)

referencing

fertilization

PMID 24614230