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In situ hybridization analysis of Spry1 and Spry2 expression domains compared to Wnt reporter activity in TCF/Lef-lacZ embryos at the stages indicated. Transverse sections are shown, dorsal oriented to the top. a – cSpry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity at early somite stages in the posterior PPR. Arrowhead indicates the presumptive otic/epibranchial region. Little or no TCF/Lef-lacZ reporter activity is detected in the posterior PPR at this stage (c). (d – f’) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (d – f) and posterior (d’ – f’) transverse sections through the OEPD. The entire OEPD is bracketed. (g – i”) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (g – i), medial (g’ – i’), and posterior (g” – i”) transverse sections through the otic placode (bracketed). | In situ hybridization analysis of Spry1 and Spry2 expression domains compared to Wnt reporter activity in TCF/Lef-lacZ embryos at the stages indicated. Transverse sections are shown, dorsal oriented to the top. a – cSpry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity at early somite stages in the posterior PPR. Arrowhead indicates the presumptive otic/epibranchial region. Little or no TCF/Lef-lacZ reporter activity is detected in the posterior PPR at this stage (c). (d – f’) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (d – f) and posterior (d’ – f’) transverse sections through the OEPD. The entire OEPD is bracketed. (g – i”) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (g – i), medial (g’ – i’), and posterior (g” – i”) transverse sections through the otic placode (bracketed). | ||
Abbreviations: Sprouty1 (Spry1), Sprouty2 (Spry2), pan-placodal region (PPR), neural ectoderm (ne), endoderm (ed), hindbrain (hb). Scale bar, 50 μm | Abbreviations: Sprouty1 (Spry1), Sprouty2 (Spry2), pan-placodal region (PPR), otic-epibranchial progenitor domain (OEPD), neural ectoderm (ne), endoderm (ed), hindbrain (hb). Scale bar, 50 μm | ||
Revision as of 09:21, 27 October 2015
Comparison of Spry1, Spry2, and Wnt reporter expression domains from pan-placodal region to otic placed stages
All cranial placodes, including the otic placode, originate from the pan-placodal region (PPR)
In situ hybridization analysis of Spry1 and Spry2 expression domains compared to Wnt reporter activity in TCF/Lef-lacZ embryos at the stages indicated. Transverse sections are shown, dorsal oriented to the top. a – cSpry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity at early somite stages in the posterior PPR. Arrowhead indicates the presumptive otic/epibranchial region. Little or no TCF/Lef-lacZ reporter activity is detected in the posterior PPR at this stage (c). (d – f’) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (d – f) and posterior (d’ – f’) transverse sections through the OEPD. The entire OEPD is bracketed. (g – i”) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (g – i), medial (g’ – i’), and posterior (g” – i”) transverse sections through the otic placode (bracketed).
Abbreviations: Sprouty1 (Spry1), Sprouty2 (Spry2), pan-placodal region (PPR), otic-epibranchial progenitor domain (OEPD), neural ectoderm (ne), endoderm (ed), hindbrain (hb). Scale bar, 50 μm
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Reference
<pubmed>6443994</pubmed>| BMC Developmental Biology
Copyright
© 2015 Wright et al. Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Wright et al. BMC Developmental Biology 2015 15:33 doi:10.1186/s12861-015-0083-8
S12861-015-0083-8-1.jpg
Cite this page: Hill, M.A. (2024, June 20) Embryology Mouse otic placode gene expression 01.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Mouse_otic_placode_gene_expression_01.jpg
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
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