File:Mouse bladder development E12.5-E16.5.jpg: Difference between revisions

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(==Schematic of depicting urogenital organs adjacent to the limb and the tail at E12.5 to E16.5== A) Whole embryos from E12.5 to E16.5 B) {{HE}} sections from E12.5 to E16.5 C) Bladder developmental progression. b: bladder, bw: body wall, u: urethra...)
 
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© 2013 Islam et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
© 2013 Islam et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


PLoS One. 2013 Apr 19;8(4):e61340. doi: 10.1371/journal.pone.0061340. Print 2013.
Spatio-temporal distribution of Smads and role of Smads/TGF-β/BMP-4 in the regulation of mouse bladder organogenesis.
Islam SS, Mokhtari RB, Kumar S, Maalouf J, Arab S, Yeger H, Farhat WA.
Source
Developmental and Stem Cell Biology, The Hospital for Sick Children Research Institute, Toronto, ON, Canada.
Abstract
Although Shh, TGF-β and BMP-4 regulate radial patterning of the bladder mesenchyme and smooth muscle differentiation, it is not known what transcription factors, local environmental cues or signaling cascades mediate bladder smooth muscle differentiation. We investigated the expression patterns of signaling mediated by Smad2 and Smad3 in the mouse embryonic bladder from E12.5 to E16.5 by using qRT-PCR, in situ hybridization and antibodies specifically recognizing individual Smad proteins. The role of Smad2 and Smad3 during smooth muscle formation was examined by disrupting the Smad2/3 signaling pathway using TβR1 inhibitor SB-431542 in organ culture system. qRT-PCR results showed that R-Smads, Co-Smad and I-Smads were all expressed during bladder development. RNA ISH for BMP-4 and immunostaining of TGF-β1 showed that BMP-4 and TGF-β1 were expressed in the transitional epithelium, lamina propia and muscularis mucosa. Smad1, Smad5 and Smad8 were first expressed in the bladder epithelium and continued to be expressed in the transitional epithelium, muscularis mesenchyme and lamina propia as the bladder developed. Smad2, Smad3 and Smad4 were first detected in the bladder epithelium and subsequently were expressed in the muscularis mesenchyme and lamina propia. Smad6 and Smad7 showed overlapping expression with R-Smads, which are critical for bladder development. In bladder explants (E12.5 to E16.5) culture, Smad2 and Smad3 were found localized within the nuclei, suggesting critical transcriptional regulatory effects during bladder development. E12.5 to E16.5 bladders were cultured with and without TβR1 inhibitor SB-431542 and assessed by qRT-PCR and immunofluorescence. After three days in culture in SB-431542, α-SMA, Smad2 and Smad3 expressions were significantly decreased compared with controls, however, with no significant changes in the expression of smooth muscle myosin heavy chain (SM-Myh. Based on the Smad expression patterns, we suggest that individual or combinations of Smads may be necessary during mouse bladder organogenesis and may be critical mediators for bladder smooth muscle differentiation.
PMID 23620745


Figure 1. doi:10.1371/journal.pone.0061340.g001
Figure 1. doi:10.1371/journal.pone.0061340.g001


[[Category:Mouse]] [[Category:Mouse E12.5]] [[Category:Mouse E14.5]] [[Category:Mouse E16.5]]
[[Category:Mouse]] [[Category:Mouse E12.5]] [[Category:Mouse E14.5]] [[Category:Mouse E16.5]]

Latest revision as of 01:24, 1 December 2013

Schematic of depicting urogenital organs adjacent to the limb and the tail at E12.5 to E16.5

A) Whole embryos from E12.5 to E16.5

B) (Stain - Haematoxylin Eosin) sections from E12.5 to E16.5

C) Bladder developmental progression. b: bladder, bw: body wall, u: urethra, gt: genital tubercle, up: urethral plate, r: rectuml, limb, t: tail, k: kidney.


Links: Urinary Bladder Development | Smooth Muscle Development | Mouse Development

Reference

<pubmed>23620745</pubmed>| PLoS One.


Copyright

© 2013 Islam et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Figure 1. doi:10.1371/journal.pone.0061340.g001

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