File:Blastocyst pluripotency markers 01.jpg: Difference between revisions
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* '''a''' - red alkaline phosphatase (AP) activity. | * '''a''' - red alkaline phosphatase (AP) activity. | ||
* '''b''' - | * '''b''' - green Oct-4 expression. | ||
* '''c''' - green TROMA-1 expression (cytokeratin 8, Endo A; cytokeratin-like filaments present in trophectoderm and endodermal cells) | * '''c''' - green TROMA-1 expression (cytokeratin 8, Endo A; cytokeratin-like filaments present in trophectoderm and endodermal cells) | ||
Latest revision as of 12:03, 5 May 2013
Localization of Pluripotency Markers
Hatched mouse blastocysts alkaline phosphatase activity and Oct-4 are markers for pluripotency, while TROMA-1 is a marker for trophectoderm specification.
- a - red alkaline phosphatase (AP) activity.
- b - green Oct-4 expression.
- c - green TROMA-1 expression (cytokeratin 8, Endo A; cytokeratin-like filaments present in trophectoderm and endodermal cells)
blue - DAPI was used for nuclear DNA counterstaining. Scale = 30 μm.
- Links: Blastocyst | Week 1 | Stem Cells | Trophoblast
Reference
<pubmed>16681851</pubmed>| PMC1479373 | J Transl Med.
Copyright
© 2006 Tanaka et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Figure 5. 1479-5876-4-20-5-l.jpg
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current | 11:25, 5 May 2013 | 1,197 × 371 (54 KB) | Z8600021 (talk | contribs) | ==Localization of pluripotency markers== Alkaline phosphatase (AP) activity (left), Oct-4 (center), and TROMA-1 (right) expression as measured in expanded and hatched blastocysts (top row, a-c) by immunofluorescent analysis. * a - red alkaline phosph... |
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