File:Blastocyst pluripotency markers 01.jpg: Difference between revisions

Localization of pluripotency markers

Alkaline phosphatase (AP) activity (left), Oct-4 (center), and TROMA-1 (right) expression as measured in expanded and hatched blastocysts (top row, a-c) by immunofluorescent analysis.

• a - red alkaline phosphatase (AP) activity.
• b - red Oct-4 expression.
• c - green TROMA-1 expression

blue - DAPI was used for nuclear DNA counterstaining. Scale = 30 μm.

Reference

Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model. Tanaka N, Takeuchi T, Neri QV, Sills ES, Palermo GD. J Transl Med. 2006 May 8;4:20. PMID: 16681851

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1479373/

http://www.translational-medicine.com/content/4/1/20

Copyright

© 2006 Tanaka et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 5. 1479-5876-4-20-5-l.jpg