User:Z3372817
Lab Attendance
Lab 1 --Z3372817 (talk) 12:45, 6 August 2014 (EST) Lab 2--Z3372817 (talk) 11:10, 13 August 2014 (EST)
http://www.ncbi.nlm.nih.gov/pubmed
Lab Assessment 1
Your Lab assessment now requires you to find a 2 recent research references on fertilisation or in vitro fertilisation. Paste each reference on your page, as shown in the class. Write below each reference a brief summary of the research article methods and findings. The summary for each need not be more than 3-4 paragraphs in length.
Research Article 1:
<pubmed>24992752</pubmed> This article tests the effect of traditional Chinese herbs on infertile women. The method employed was to conduct tests on 433 infertile women below the age of 42 and dividing the groups into test subjects, those who will be administered Chinese herbs, against the control. The groups were made up of 216 people and 217 in the respective groups. All subjects were given 1 out of 4 options of ultra-ovulation-promoting therapy to assist in the in-vitro fertilisation.
The main categories of measurement and the subsequent findings were:
| Category | Result |
|---|---|
| Endometrium thickness | Higher than control |
| Number of acquired eggs | No difference with control |
| Rates of normal fertility | Higher than control |
| High quality embryos | Higher than control |
| Biochemical and clinical pregnancy rate of subjects | Higher than control |
The findings showed an overall improved quality of fertility in these otherwise infertile women of the intervention group. The embryos also exhibited increased quality. This finding then suggested an improved success rate of IVF-embryo transplantation cycles and increased outcomes and safety of assisted reproductive technology.
Research Article 2:
<pubmed>23835722</pubmed> The effect of two different oxygen levels on embryo development was tested. The female gametes (oocyte) of 258 women were divided in a randomised study into 2 different groups; incubator of 5% oxygen concentration versus an incubator of 20% oxygen concentration. The purpose of the incubator is to ensure oxygen concentration is constant throughout the course of the experiment.
The matters of interest along with the clinical outcomes are as follows:
| Category | Result |
|---|---|
| Fertilisation | Same between groups |
| Cleavage | Same between groups |
| Embryo quality | Higher in 5% conc. group
(more blastomeres, more cycles of favourable embryos) |
| Blastocyst formation | Higher in 5% conc. group |
| Implantation | Higher in 5% conc. group |
| Pregnancy | Higher in 5% conc. group |
| Live birth rates | Higher in 5% conc. group |
The findings showed higher, greater quality embryos were seen in test subjects of the 5% oxygen concentration group. Smaller oxygen levels in incubation during embryo development was more favourable.
