User:Z3372817

Lab Attendance

Lab 1 --Z3372817 (talk) 12:45, 6 August 2014 (EST)

Lab 2 --Z3372817 (talk) 11:10, 13 August 2014 (EST)

http://www.ncbi.nlm.nih.gov/pubmed

PubMed

PMID2508416

Lab Assessment 1

Your Lab assessment now requires you to find a 2 recent research references on fertilisation or in vitro fertilisation. Paste each reference on your page, as shown in the class. Write below each reference a brief summary of the research article methods and findings. The summary for each need not be more than 3-4 paragraphs in length.

Research Article 1:

<pubmed>24992752</pubmed> This article tests the effect of traditional Chinese herbs on infertile women. The method employed was to conduct tests on 433 infertile women below the age of 42 and dividing the groups into test subjects, those who will be administered Chinese herbs, against the control. The groups were made up of 216 people and 217 in the respective groups. All subjects were given 1 out of 4 options of ultra-ovulation-promoting therapy to assist in the in-vitro fertilisation.

The main categories of measurement and the subsequent findings were:

Category	Result
Endometrium thickness	Higher than control
Number of acquired eggs	No difference with control
Rates of normal fertility	Higher than control
High quality embryos	Higher than control
Biochemical and clinical pregnancy rate of subjects	Higher than control

The findings showed an overall improved quality of fertility in these otherwise infertile women of the intervention group. The embryos also exhibited increased quality. This finding then suggested an improved success rate of IVF-embryo transplantation cycles and increased outcomes and safety of assisted reproductive technology.

Research Article 2:

<pubmed>23835722</pubmed> The effect of two different oxygen levels on embryo development was tested. The female gametes (oocyte) of 258 women were divided in a randomised study into 2 different groups; incubator of 5% oxygen concentration versus an incubator of 20% oxygen concentration. The purpose of the incubator is to ensure oxygen concentration is constant throughout the course of the experiment.

The matters of interest along with the clinical outcomes are as follows:

Category	Result
Fertilisation	Same between groups
Cleavage	Same between groups
Embryo quality	Higher in 5% conc. group (more blastomeres, more cycles of favourable embryos)
Blastocyst formation	Higher in 5% conc. group
Implantation	Higher in 5% conc. group
Pregnancy	Higher in 5% conc. group
Live birth rates	Higher in 5% conc. group

The findings showed higher, greater quality embryos were seen in test subjects of the 5% oxygen concentration group. Smaller oxygen levels in incubation during embryo development was more favourable.

Lab Assessment 2

IHC mice fetal lung tissue image showing the role of GAD and GABA in respiratory fetal development

Reference

Chintagari NR, Jin N, Gao L, Wang Y, Xi D, et al. (2010) Role of GABA Receptors in Fetal Lung Development in Rats. PLoS ONE 5(11): e14171. doi:10.1371/journal.pone.0014171 | PLoS One: Role of GABA Receptors in Fetal Lung Development in Rats

© 2010 Chintagari et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

---

Note - This image was originally uploaded as part of an undergraduate science student project and may contain inaccuracies in either description or acknowledgements. Students have been advised in writing concerning the reuse of content and may accidentally have misunderstood the original terms of use. If image reuse on this non-commercial educational site infringes your existing copyright, please contact the site editor for immediate removal.