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Revision as of 16:22, 31 July 2012

Lab Attendance

Lab 1

--Z3332863 11:47, 25 July 2012 (EST)

Origin of Nobel Prize & Discoverer

In 2010, Robert G. Edwards won the Nobel Prize for developing In vitro Fertilisation. IVF originated in 1950s when Edwards began fertilizing human eggs in cell culture dishes as a way of treating infertility. In 1978, Edward's IVF technology gave the world's first IVF baby. Over the next few years, Edwards and his team fine-tuned the technique of IVF.

[1]

Research paper on fertilisation:

What does this paper tell us about fertilisation?

This article looks at the rise of aneuploidies in IVF embryos from women around 40yrs of age. To do this Handyside et al, used 'microarray comparative genomic hybridisation' technology to study the chromosome copy number in the zygote, the 1st and 2nd polar bodies in older women receiving IVF treatment. Handyside et al found that:

72% of oocytes examined had aneuploidies in either one or both of the polar bodies.
Most of the aneuploidies of IVF embryos arose from the 2nd meiotic division of the oocyte. This is surprising because most aneuploidies in naturally fertilized embryos arise from Meiosis I of the oocyte.
Aneuploidies in IVF zygotes were not due to non-disjunction of chromosomes in the oocyte. Instead, these Aneuploidies were due to predivision of the chromatids.
In IVF zygotes made from aged oocytes, often there were multiple aneuploidies in 1 zygote.

By looking at the origin of anueploidies in zygotes obtained by IVF, these scientists are trying to find a way to reduce these aneuploidies.

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 '''What does this paper tell us about fertilisation?'''
-This article looks at the rise of aneuploidies in IVF embryos in older women. To do this Handyside et al, used 'microarray comparative genomic hybridisation' technology to study the chromosome copy number of the zygote, the 1st and 2nd polar bodies in aged women receiving IVF treatment. Handyside et al, found that unlike natural fertilization, most of the aneuploidies of IVF embryos arose from the 2nd meiotic division, not the first. They also found the IVF aneuploidies that did occur in the first meiotic division, were not due to non-disjunction. Instead, these Aneuploidies were due to premature predivision of the chromatids. In aged zygotes, often multiple aneuploidies were found.
+This article looks at the rise of aneuploidies in IVF embryos from women around 40yrs of age. To do this Handyside et al, used 'microarray comparative genomic hybridisation' technology to study the chromosome copy number in the zygote, the 1st and 2nd polar bodies in older women receiving IVF treatment. Handyside et al found that:
+* 72% of oocytes examined had aneuploidies in either one or both of the polar bodies.
+* Most of the aneuploidies of IVF embryos arose from the 2nd meiotic division of the oocyte. This is surprising because most aneuploidies in naturally fertilized embryos arise from Meiosis I of the oocyte.
+* Aneuploidies in IVF zygotes were not due to non-disjunction of chromosomes in the oocyte. Instead, these Aneuploidies were due to predivision of the chromatids.
+* In IVF zygotes made from aged oocytes, often there were  multiple aneuploidies in 1 zygote.
+By looking at the origin of anueploidies in zygotes obtained by IVF, these scientists are trying to find a way to reduce these aneuploidies.
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