Talk:Bovine Oocyte Transport Movie

From Embryology

Lipopolysaccharide (LPS) disrupts particle transport, cilia function and sperm motility in an ex vivo oviduct model

Sci Rep. 2016 Apr 15;6:24583. doi: 10.1038/srep24583.

O'Doherty AM1, Di Fenza M1, Kölle S1.


The oviduct functions in the transportation of gametes to the site of fertilization (the ampulla) and is the site of early embryonic development. Alterations of this early developmental environment, such as the presence of sexually transmitted pathogens, may affect oviduct function leading to reduced fertilization rates and contribute to compromised embryonic development. In this study, sperm interactions, particle transport speed (PTS) and cilia beat frequency (CBF) in the ampulla following exposure to lipopolysaccharide (LPS), a constituent of the sexually transmitted pathogens Chlamydia trachomatis and Chlamydia abortus, was investigated. Three complementary experiments were performed to analyse; (1) bound sperm motility and cilia function (2) transport velocity in the oviduct and (3) the expression of genes related to immune function and inflammatory response (CASP3, CD14, MYD88, TLR4 and TRAF6). The motility of bound sperm was significantly lower in ampullae that were exposed to LPS. CBF and PTS significantly increased after treatment with LPS for 2 hours. Finally, gene expression analysis revealed that CASP3 and CD14 were significantly upregulated and TLR4 trended towards increased expression following treatment with LPS. These findings provide an insight on the impact of LPS on the oviduct sperm interaction, and have implications for both male and female fertility. PMID 27079521 PMCID: PMC4832340 DOI: 10.1038/srep24583


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80: Kolle SN, Sullivan KM, Mehling A, van Ravenzwaay B, Landsiedel R. Applicability of in vitro tests for skin irritation and corrosion to regulatory classification schemes: substantiating test strategies with data from routine studies. Regul Toxicol Pharmacol. 2012 Dec;64(3):402-14. doi: 10.1016/j.yrtph.2012.08.015. Corrected and republished in: Regul Toxicol Pharmacol. 2013 Apr;65(3):366-78. PubMed PMID 22939940.

81: Mehling A, Eriksson T, Eltze T, Kolle S, Ramirez T, Teubner W, van Ravenzwaay B, Landsiedel R. Non-animal test methods for predicting skin sensitization potentials. Arch Toxicol. 2012 Aug;86(8):1273-95. doi: 10.1007/s00204-012-0867-6. Review. PubMed PMID 22707154.

82: Bauch C, Kolle SN, Ramirez T, Eltze T, Fabian E, Mehling A, Teubner W, van Ravenzwaay B, Landsiedel R. Putting the parts together: combining in vitro methods to test for skin sensitizing potentials. Regul Toxicol Pharmacol. 2012 Aug;63(3):489-504. doi: 10.1016/j.yrtph.2012.05.013. Erratum in: Regul Toxicol Pharmacol. 2012 Nov;64(2):285. PubMed PMID 22659254.

83: Kolle SN, Basketter D, Schrage A, Gamer AO, van Ravenzwaay B, Landsiedel R. Further experience with the local lymph node assay using standard radioactive and nonradioactive cell count measurements. J Appl Toxicol. 2012 Aug;32(8):597-607. doi: 10.1002/jat.2754. PubMed PMID 22627909.

84: Kolle SN, Ramirez T, Kamp HG, Buesen R, Flick B, Strauss V, van Ravenzwaay B. A testing strategy for the identification of mammalian, systemic endocrine disruptors with particular focus on steroids. Regul Toxicol Pharmacol. 2012 Jul;63(2):259-78. doi: 10.1016/j.yrtph.2012.04.004. PubMed PMID 22554500.

85: Kolle SN, Kandárová H, Wareing B, van Ravenzwaay B, Landsiedel R. In-house validation of the EpiOcular(TM) eye irritation test and its combination with the bovine corneal opacity and permeability test for the assessment of ocular irritation. Altern Lab Anim. 2011 Sep;39(4):365-87. PubMed PMID 21942548.

86: Schrage A, Hempel K, Schulz M, Kolle SN, van Ravenzwaay B, Landsiedel R. Refinement and reduction of acute oral toxicity testing: a critical review of the use of cytotoxicity data. Altern Lab Anim. 2011 Jul;39(3):273-95. Review. PubMed PMID 21777041.

87: Bauch C, Kolle SN, Fabian E, Pachel C, Ramirez T, Wiench B, Wruck CJ, van Ravenzwaay B, Landsiedel R. Intralaboratory validation of four in vitro assays for the prediction of the skin sensitizing potential of chemicals. Toxicol In Vitro. 2011 Sep;25(6):1162-8. doi: 10.1016/j.tiv.2011.05.030. PubMed PMID: 21669280.

88: Ball N, Cagen S, Carrillo JC, Certa H, Eigler D, Emter R, Faulhammer F, Garcia C, Graham C, Haux C, Kolle SN, Kreiling R, Natsch A, Mehling A. Evaluating the sensitization potential of surfactants: integrating data from the local lymph node assay, guinea pig maximization test, and in vitro methods in a weight-of-evidence approach. Regul Toxicol Pharmacol. 2011 Aug;60(3):389-400. doi: 10.1016/j.yrtph.2011.05.007. PubMed PMID 21645576.

89: Kolle SN, Melching-Kollmuss S, Krennrich G, Landsiedel R, van Ravenzwaay B. Assessment of combinations of antiandrogenic compounds vinclozolin and flutamide in a yeast based reporter assay. Regul Toxicol Pharmacol. 2011 Aug;60(3):373-80. doi: 10.1016/j.yrtph.2011.05.005. PubMed PMID 21620918.

90: Basketter D, Kolle SN, Schrage A, Honarvar N, Gamer AO, van Ravenzwaay B, Landsiedel R. Experience with local lymph node assay performance standards using standard radioactivity and nonradioactive cell count measurements. J Appl Toxicol. 2012 Aug;32(8):590-6. doi: 10.1002/jat.1684. PubMed PMID 21618258.

91: Schrage A, Kolle SN, Moreno MC, Norman K, Raabe H, Curren R, van Ravenzwaay B, Landsiedel R. The bovine corneal opacity and permeability test in routine ocular irritation testing and its improvement within the limits of OECD test guideline 437. Altern Lab Anim. 2011 Mar;39(1):37-53. PubMed PMID 21452913.

92: Balcke GU, Kolle SN, Kamp H, Bethan B, Looser R, Wagner S, Landsiedel R, van Ravenzwaay B. Linking energy metabolism to dysfunctions in mitochondrial respiration--a metabolomics in vitro approach. Toxicol Lett. 2011 Jun 24;203(3):200-9. doi: 10.1016/j.toxlet.2011.03.013. PubMed PMID 21402135.

93: Kolle SN, Kamp HG, Huener HA, Knickel J, Verlohner A, Woitkowiak C, Landsiedel R, van Ravenzwaay B. In house validation of recombinant yeast estrogen and androgen receptor agonist and antagonist screening assays. Toxicol In Vitro. 2010 Oct;24(7):2030-40. doi: 10.1016/j.tiv.2010.08.008. PubMed PMID 20732407.

94: Rohe T, Becker W, Kölle S, Eisenreich N, Eyerer P. Near infrared (NIR) spectroscopy for in-line monitoring of polymer extrusion processes. Talanta. 1999 Sep 13;50(2):283-90. PubMed PMID 18967719.

95: Kolle S, Mørkve O. [Diagnosis and treatment of obstructive lung diseases]. Tidsskr Nor Laegeforen. 1998 Sep 10;118(21):3268-71. Norwegian. PubMed PMID: 9772813.

96: Jagla W, Wiede A, Kölle S, Hoffmann W. Differential expression of the TFF-peptides xP1 and xP4 in the gastrointestinal tract of Xenopus laevis. Cell Tissue Res. 1998 Jan;291(1):13-8. PubMed PMID 9394039.