Talk:2009 Group Project 3

ZebraFish - Zebrafish are really cool...

I'm happy with zebrafish... never heard of them but they sound interesting :) what does everyone else think? ...Gaby Pinget

Great, who else is in our group then... Oh and i'm Sal by the way

Ok so i'm not sure who the other two are because i don't know your number but just for reference my number is z3218657. I found this cool website with some pictures of Zebrafish embryo's developing... its pretty cool...

http://www.cas.vanderbilt.edu/bioimages/animals/danrer/zfish-devel.htm

and this website shows all the stages and times and such good for a timeline

http://zfin.org/zf_info/zfbook/stages/stages.html

Research Topics for this week:

- Timeline and Staging (Sal)
- History of Model Use (Gaby)
- Genetics (Bronwyn)
- Current Embryology Research (Jo)

--> Share what we've learnt with the group next session.

Hey Guys I have a lot of info... and i don't think i should post it all up here because there is a lot of stuff... Mainly all about the Staging though. I am going to put up a summary of the different stages. The website i gave you before ZFIN is the zebrafish database of model organisms. its great!

Zygote Period – Lasts for 0-0.75hours Transition of one cell to two cells. Fertilisation occurs activating cytoplastimic movement. The animal poles within the cell segregate the blastodisc from the yolk cytoplasm. Segregation continues into the cleavage staging.

Cleavage Period – Lasts for 0.75-2.25 hours Transition from two cells to 128 cells After the first cleavage the blastocysts division is approx every 15 minutes.

Blastula Period – Lasts for 2.25-5.25 hours Transition from 128 cells to 50% epibolby “Epiboly, beginning in the late blastula (Solnica-Krezel and Driever, 1994), is the thinning and spreading of both the YSL and the blastodisc over the yolk cell, as you might model by pulling a knitted ski cap over your head” – Direct quote from the ZFIN website

Gastrula Period – Lasts for 5.25-10.33 hours Transition from 50% epibolby to 1-4 somites The gastrula period ends when epiboly is complete, and the tail bud has formed. Here each germ layer (endoderm, mesoderm, ectoderm) is put in the right place so that bodily organs and tissues can form in the correct locations.

Segmentation period – Lasts from 10.33 – 24 hours Transistion from 1-4 somites to Prim-5 Here dermis, vertebrae and skeletal muscle are formed

Pharyngula Period – Lasts from 24 – 48 hours Transition from Prim 5 to Long-pec The body axis begins to straighten and the fins begin to develop.

http://www.youtube.com/watch?v=0hGT667ktTw

Hatching Peroid – Last from 48 to 72hours Transitions from Long-Pec to Protruding-mouth In this period, primary organ systems develop and cartilage development begins.

Larval Period – Lasts from 72hours to 30days transition from Protruding-mouth to Day 30-44 The pectoral fin continues to develop and the internal organs become more complex. Development continues.

Juvenille Period – Lasts from 30-44 days Here adult fins and pigments as well as 12 teeth develop.

AdultPeriod – Lasts90days to 2 years Full Breeding Adult.

http://www.youtube.com/watch?v=5ygcu9BRXI0 - Zebrafish heart beating!

http://www.youtube.com/watch?v=TbErcmhzUSY - alcohol effects on Zebrafish embryo

Websites used http://dev.biologists.org/cgi/content/abstract/dev.022673v1

http://www.zfic.org/classroom%20experiments/stagingindex.html

http://zfin.org/zf_info/zfbook/stages/stages.html

Hey! This is a really good site with a bunch of links relating to the different areas we have to research: http://www.sanger.ac.uk/modelorgs/zebrafish.shtml

Hey guys.... Ive found a really good picture of the zebrafish embryo development (like the human one) but I dont know how to put it up, was thinking it might be better if it was actually on the page rather than a link to get the picture. JO

well I think that I just uploaded a picture but I have no idea where to... wow I'm so lost... Maybe that's a good thing because I have a feeling that it was copy right protected... DAMN COPY RIGHT ah ha ha

Hey all, all the websites that i have put up on this page have really good images... I have emailed the three of them and asked if it was ok if i used them to put up on here and i am awaiting a reply so hopefully they will let us use them. Also i am having a tonne and a half of trouble uploading a InDesign Timeline image i created of the timeline and also having some issues with the net so i might bring them to class and see if the computers there are readable other wise i am screwed and will just have to start all over again. I have a more complete Timeline and Staging format that i have created as well. I'll put that up when i try and upload the image again. Peace.

Hey Hey So I got permission from Judy Cebra-Thomas to use all her images on her website which are each stage of development - YAY we just have to accredit them. So today apart from working on unwinding my InDesign TImeline and putting the info up in bullets i am going to start to figure out whats happening in all the images and try an upload them onto the site so hopefully tomorrow we will have an almost finished timeline and staging section!

Right so I've posted a link to the current sequencing status on the genetics part of our site. This updates pretty much everyday so I will be able to update ours right before submission so we have up-to-date info. This is however a minor detail so I'm writing it here to help us all remember! :) thanks