Talk:2009 Group Project 3

ZebraFish - Zebrafish are really cool...

I'm happy with zebrafish... never heard of them but they sound interesting :) what does everyone else think? ...Gaby Pinget

Great, who else is in our group then... Oh and i'm Sal by the way

Ok so i'm not sure who the other two are because i don't know your number but just for reference my number is z3218657. I found this cool website with some pictures of Zebrafish embryo's developing... its pretty cool...

http://www.cas.vanderbilt.edu/bioimages/animals/danrer/zfish-devel.htm

and this website shows all the stages and times and such good for a timeline

http://zfin.org/zf_info/zfbook/stages/stages.html

Research Topics for this week:

- Timeline and Staging (Sal)
- History of Model Use (Gaby)
- Genetics (Bronwyn)
- Current Embryology Research (Jo)

--> Share what we've learnt with the group next session.

Hey Guys I have a lot of info... and i don't think i should post it all up here because there is a lot of stuff... Mainly all about the Staging though. I am going to put up a summary of the different stages. The website i gave you before ZFIN is the zebrafish database of model organisms. its great!

Zygote Period – Lasts for 0-0.75hours Transition of one cell to two cells. Fertilisation occurs activating cytoplastimic movement. The animal poles within the cell segregate the blastodisc from the yolk cytoplasm. Segregation continues into the cleavage staging.

Cleavage Period – Lasts for 0.75-2.25 hours Transition from two cells to 128 cells After the first cleavage the blastocysts division is approx every 15 minutes.

Blastula Period – Lasts for 2.25-5.25 hours Transition from 128 to 50% epibolby “Epiboly, beginning in the late blastula (Solnica-Krezel and Driever, 1994), is the thinning and spreading of both the YSL and the blastodisc over the yolk cell, as you might model by pulling a knitted ski cap over your head” – Direct quote from the ZFIN website

Gastrula Period – Lasts for 5.25-10.33 hours Transition from 50% epibolby to 1-4 somites The gastrula period ends when epiboly is complete, and the tail bud has formed. Here each germ layer (endoderm, mesoderm, ectoderm) is put in the right place so that bodily organs and tissues can form in the correct locations.

Segmentation period – Lasts from 10.33 – 24 hours Transistion from 1-4 somites to Prim-5 Here dermis, vertebrae and skeletal muscle are formed

Pharyngula Period – Lasts from 24 – 48 hours Transition from Prim 5 to Long-pec The body axis begins to straighten and the fins begin to develop.

Hatching Peroid – Last from 48 to 72hours Transitions from Long-Pec to Protruding-mouth In this period, primary organ systems develop and cartilage development begins.

Larval Period – Lasts from 72hours to 30days transition from Protruding-mouth to Day 30-44 The pectoral fin continues to develop and the internal organs become more complex. Development continues.

Juvenille Period – Lasts from 30-44 days Here adult fins and pigments as well as 12 teeth develop.

AdultPeriod – Lasts90days to 2 years Full Breeding Adult.

Websites used http://dev.biologists.org/cgi/content/abstract/dev.022673v1

http://www.zfic.org/classroom%20experiments/stagingindex.html

http://zfin.org/zf_info/zfbook/stages/stages.html