View source for Talk:1987 Developmental Stages In Human Embryos - Appendix 1


Carnegie Collection stage 13 table

Carnegie Collection stage 14 table


==Stage 6==

Serial
No. Grade
Embedding Medium
? P
P
P
P C-P
STAGE 6 Thinness
6026 Poor 6734 Poor 6900 Poor 7634 Poor 7762 Good
7800 Exc.
7801 Exc.
7850 Exc.
8290 Exc.
8360 Exc.
8362 Poor p
6? 10
15 10 8 8 8 6 8 6 6 6 8 6 8
H.&E.
H.&E.
H.&E.
H.&E.
?
H.&E.
H.&E.
H.&E.
H. phlox. H.&E., phlox. H.&E., phlox H.&E.
H.&E.
H.&E., phlox. H.&E., phlox. Azan
8672 Exc.
8819 Exc.
8905 Poor
8910 Good Formol 9222 Good Bouin 9250 Exc. Bouin 9595 Poor p
10003 Good Bouin
6& 10
8 H.&E. 1954 8 H.&E. 1958 5 V arious 1963
Fixative
? Zenker-acetic Formol Formol Zenker-formol
Stain
Y ear Notes
1929 Lockyer embryo. Abnormal. Ramsey (1937) 1934 Yale embryo. Ramsey (1938)
1940 Linzenmeier (1914)
1940 Torpin embryo. Krafka (1941)
1940 Wilson (1945)
1940 Abnormal
1940 Heuser et al. (1945)
1940 Abnormal
1944
1944
1944
1949
1951 Edwards-Jones-Brewer (H.I496). Brewer (1938) 1951 Abnormal
1951
1954 Abnormal. Possibly stage 7
Bouin C-P Ale. & Bouin C-P Bouin C-P
Ale. & Bouin
C-P C-P C-P C-P p C-P C-P p
p p
Ale. & Bouin Formol-chrom. subl. Ale.
etc.

==STAGE 7==
Serial Embedding Thinness
No. Grade Fixative Medium ((xm) Scain
Y ear
1940 1943 1946 1948 1950 1950 1954
Notes
Heuser et al. (1945)
Abnormal
7802 Exc. Ale. & Bouin 8206 Good p
8361 Good Bouin
8602 Exc. Ale.
8752 Exc. 7
8756 Exc. Formol 9217 Exc. p
C-P 6 H.&E. C-P 6 H.&E. C-P 10 p
C-P 8 H.&E. C-P 10 H&E C-P 10 H&E P10 H.&E.

==STAGE 8==

Serial No.
1399 3412
5960
6630
6815
7170a and b 7545
7568 7640 7666
7701 7822 7949 7972 8255
8320 8352 8371 8671 8725 8727
8820
9009a and b 9123
9251
9286
10157 10174
Embedding Grade Fixative Medium
Poor Formol P Poor Formol P
Good Kaiserling P Poor Formol P Poor Formol P Poor Ale. C-P
Plane
Trans. Trans.
Trans. Oblique Oblique Trans. Trans. Trans. Trans. Trans.
Trans. Trans. Sag. Sag. Sag.
Sag. Trans. Sag. Sag. Sag. Trans.
Trans.
Sag. Sag. Trans. Trans. Trans.
Thinness
(fxm) Stain Year Notes
Exc. Poor Good Exc.
Exc. Good Good Good Exc.
Good Good Poor Exc. Exc. Exc.
Good
Formoi C-P Formol C-P
Formol & Bouin P Formol-chrom. C-P subl.
? C-P Formol C-P Zenker p
Ale. & Bouin C-P Bouin C-P
Formol C-P Formol C-P Ale. & Bouin C-P Ale. & Bouin C-P Ale. & Bouin C-P Ale. & Bouin C-P
Zenker-formol
10 H.&E. etc.
5-15 Al. coch. E. au., or. /-*
5 Al. coch. & eosin
6 H.&E.
10 Al. coch., or. G
6 H.&E.
6 H.&E.
10 Al. coch. 10 H.&E.
6 H.&E.
1916 "Mateer embryo" described by Streeter (1920a) 1921
1929 Heuser (1932b) 1932
1933
1935 Twins
1938
1938
1939 George (1942) 1939 "H. 1515"
Formol C-P Formol C-P ? C-P C-P
8 H.&E. 1939 10 H.&E. 1940 10 H.&E. etc. 1941
6 H.&E. 1942 8 H.&E., phlox. 1944
8 H.&E., phlox. 1945 8 H.&E., phlox. 1946 8 H.&E., phlox. 1946 6 H.&E, phlox. 1949 6 H.&E, phlox. 1949 8 H.&E, phlox. 1949
10 H.&E. 1951
6 H.&E. 1952
6 H.&E. 1953 10-12 Azan, H. & phlox. 1954 8 Azan 1955 ? Cason 1967 8 Cason 1967
Slides showing embryo returned to Dr. Patten in 1962
Germ disc folded, possibly double (Hertig, 1968, fig. 180)
"Jones-Brewer I" (H. 1459) described byJones and Brewer (1941)
Twins described briefly by Heuser (1954)
Good
Good
Good
Exc. Formol Exc. Formol Exc. Bouin
p
p Trans.

==STAGE 9==

Serial No.
1878 5080 7650
Pairs of somites
2-3 1
2-3
Size(mm)
E., 1.38
Ch., 12X10.5X7.5 E., 1.5
Ch., 14.5
E., 2-3
Grade Fixative Good Formol
Poor Formol Good Ale. & Bouirl
Medium Plane P Cor.
P Trans. C-P Trans.
(u-m) Stain Year 10 H.&E. 1917
10 Al. coch. 1926 6 H.&E. 1939
Notes Described by Ingalls (1920)
Studied by Davis (1927)
Said to be female (Park, 1957)

Embedding ness
Thin-

==STAGE 10==

Serial No.
391 1201 2795 3"T09
Paired Size somites (mm)
8 E, 2 Ch., 14
"7 E,2
Ch.. 144
Grade Fixative Gcxxl Formol Good Formol
Embedding medium
Thinness
(fim) Stain
10 Al. coch.
8 H. & or. G.
6 Alcoch,or.G. 12.5 I. H.
10 Erythrosin
Y ear 1907
1915
1919 1921 1921
Notes Monograph by Dandy (1910)
Univ. Chicago No. H 87
Univ. Calif. No. H 19"7 Univ. Chicago No H 279
4-5 E,2 Poor Ale.
12 E, 1 5 Good Formol 4 E.. 1 P(x>r Formol
Ch, 148

Serial No.
Paired Size Embedding Thinness somites (mm) Grade Fixative medium (u.m)
Stain H. & or. G.
?
Al. coch.
Ehr. H.
7
Year Notes 1921 Univ. Chicago No. H 392
1923 Monograph by Payne (1925)
1925 Univ. Rochester No. H 10. Monograph by Corner (1929)
1931 Univ. Chicago No. H 1404
1933 Litzenberg embryo. Studied by Boyden (1940) 1941 "Singapore embryo." Univ. Cambridge No. H 98.
3710 12
4216 8
5074 10
6330 7 6740 12 7251 8
8244 6 8970 12
E., 3.6 Ch., 19.0 E, 2
Ch, 9.8 E., 3.3 Ch., 10.8 E, 2.83 E., 2.2 E., 1.27
E., 1.55 Ch, 8,5 Ch, ca. 8
Good Formol Good Formol Exc. Bouin Good •>
Good p Good Formol
Good Ale. Good Zenker
C-P 10
P15
P10
C-P 5 •> 8
C-P 10 C-P 8 p5
H.&E.
H.&E, phlox. 1944
STAGE 10 (continued)
All sections at stage 10 are transverse
Various, chiefly carmine
Studied by Wilson (1914)
1952 Univ. Chicago. No. H 637. Dicephaly



==STAGE 11==

Serial Paired Size
No. somites (mm) Grade
12 14 E, 2.1 Poor Ch, 13
164 18 E, 3.5 Good Ch, 14
318 13/14 E, 2.5 Good Ch, 16
470 17 E, 4.3 Good Ch, 16
Fixative Formol
Formol
•>
Thin- Embedding ness
medium Plane P Trans.
P Trans. P Trans. P Trans. C Trans.
779 14
E, 2.75 Good
20 Al. coch. 1913 25 Al. coch. 1905 10 Al. coch. 1910 15 Al. coch.
20 Al. coch. 1915
Ch, 16x14x12
1182b E, 3 Good
1913 Complete dysraphia. Studied by Dekaban and Bartelmez (1964)
2053 20
4315 17
4529 14
4783 13 4877 13
5072 17
Ch, 15x12x5
E, 3.1 Exc. Ch, 12
E, 4.7 Exc. Ch, 23x10.4X11
E, 2.4 Exc. Ch, 21
E, 2.3 Fair E, 2 Good Ch, 15
E, 3 Good
Formol
Formol P
C-P Formol P
Formol P
Formol P
Formol C-P
Formol C-P
Formol C-P
Ale, p formol
Bouin C-P
C-P Formol C-P
Formol C-P Bouin C-P
Trans.
Trans.
Trans.
Trans.
Trans. Trans.
Trans.
Cor.
Trans.
Trans.
Oblique
Trans.
Trans. Trans
Trans.
10 Al. coch.
10 I.H. & E.
10 Al. coch, or. G.
1918 Most advanced in group. Monograph by Davis (1923). Ag added to slide 2
1923 Univ. Chicago No. 951. Studied by Wen
(1928)
1924 Monograph by Heuser (1930)
6050
6344
6784
7358
7611
7665 7702
7851
8005 8116
19/21 E.,3 Good Ch, 10
10 H.&E. 10 Al. coch. 6 Al. coch.
1925 Tubal. Type specimen. Monograph by Atwell (1930)
1930 Advanced
8962
Formol
?*Sag, 1952
Tubal. I'ntv Chicago No H 810
13
17
16
16
19 17
13
16/1"7 17
15
E, 2.5 Exc. Ch, 17
E, 5 Exc. Ch, 16
E, ? Poor Ch, 15
E., 2.4 Exc. Ch., 12
E., 4,36 Exc. E, 3.7 Good Ch., 14
E., 4.3 Exc. Ch, 18
E, 3 Exc. E, 14 Good Ch.. 17
E, 1 55 GIKXJ
1931 Least advanced in group 6 I.H, or. G. 1933

Trans
p Sag 8
1942 Tuba! Azan 1953
(u-m) Stain
10 Al. carm. 1893
5 I.H.
15 Al. coch. 1925
25 H.&E. 1936 8 H.&E. 1938
6 >
10 Al. coch.
8 H.&E. 8 H.&E.
1939 Univ. Chicago No. H 1516 1940 Returned to B M Patten
1940 Slightly injured
Year Notes
1924 Monograph by Wallin (1913)


==STAGE 12==

Thin- Serial Paired Size Embedding ness
No. somites (mm) Grade Fixative medium Plane (|im) Stain Year
Notes
209 ca.24 E.,3 Ch.,15
Poor Ale.
Poor p
Poor Formol
Good Corros. acetic Good Formol
Poor Formol Good Ale, formol Poor Formol
P Cor. 50
? Sag. 20
P Trans. 10
P Trans. 10
P Trans. 20
P Trans. 10
P Trans. 10
P Trans. 40
P Cor. 10
P Trans. 15
P Trans. 10 C-P Trans. 10
Al. coch. 1902 Al. coch. p
250 19? 384 P 486 21
E, 2
Ch., 10x9x9 E., 2.5
Ch.,13
E.,4
Ch., 22
E.,4.5
Ch., 20
E., 5.3
Ch., 19.5
H.&E.
Al. coch.
Al. coch.
Al. coch, or. G.
Al. coch.
Al. coch.
1907 Macerated. Narrow yolk stalk 1911
1062 29
2197 •>
4245-7 ca.24 E.,3.5
1915 1918 1923 1923
Ch., 24 4479 E., 5.8
C h , 17
4736 26 E.,3.0 Good Formol
Al. coch. 1924
4759
4784 5035
Ch.,20 7 E.,4.5
Ch., 15 23 E.,3
25-28 E.,3.8 Ch., 18
Good Formol
Good p Good Formol
H.&E.
5048 ca.25 E.,3.5 Good Formol C-P Trans. 10
Al. coch. 1925
Tubal. Injured
Tubal
Autopsy. Partly macerated
Tubal. Ag added to slides 1-3
Tubal. Caudal neuropore closed Caudal end broken
Typical for stage 12
Caudal defect
5056 5206 5300 5923 6097 6144 6488 6937
7724 7852 7999 8505a
25 E.,3 Good Formol Ch.,12
Trans. 10 Trans. 20 Trans. 20
C-P Trans. 10 Trans. 10 Trans. 10 Cor. 10 Sag. 8 Trans. 10 C-P Trans. 10
Al. Coch.
Al. coch.
Al. coch.
Al. coch.
Al. coch, eosin Al. coch.
Al. coch.
I. H., or. G.
H.&E.
H.&E.
H.&E.
H. Phlox. Azan
1925 1926 1926 1929 1930 1930 1932 1934 1940 1940 1942
b 23
8941 28
8942 25
8943 22
8944 25
8963 22
8964 23
9154 24
Exc Formol C-P Exc Zenker C-P
Trans. 6 Cor. 5 Trans. 8
I.H. 1927 I.H. 1930 H.&E. 1934 I.H. 1936 I.H. 1928 I.H. 1928 I.H. & phlox
Univ. Chicago No. H 1261
Univ. Chicago No. H 1382
Univ. Chicago No. H 1481
Univ. Chicago No. H 1514
Univ. Chicago. No. H 1093 Studied by Wen (1928)
Univ. Chicago No. H 984. Studied by Wen (1928)
p
p 28 25 27 28 26 ca. 29 25 ca.28 24
E.,4 Poor p Ch., 51x31x30
7
P
Exc. Formol P Trans. 10
Poor Formol
E., 4.5 Ch,16.5 E.,4 Ch.,15 E,3.4 Ch., 12.5 E. 3.3 Ch., 11 E, 3.2 Ch,22 E.,3
C h , 12 E, 3.5 Ch.,18 E , 3.7 Ch,26 E, 3.2
C h , 15 Ch, 23.5 Ch,24
E, 4.9
Ch, 35
E, 38
Ch, 35
E. 3.9
Ch,, 20,4
E,4 Exc. Formal- C-P Sag. 8 Ch,,25 Zenker
E, 3.8 Fair Formol C-P Trans. 10 Ch , 14 5
E,2.8 Pix>r Formol p Trans 8 Ch . 26 "
E, 5 4 Exc Formal C-P Trans 8&6
Exc. Formol
Good Lysol-Zenker C-P
Good Formol C-P
Poor Formol C-P
Good Formol C-P
Exc. Formol C-P
Exc. Bouin
Exc Formol C-P
Trans. p Sag. p
\ 1 CiA~J
( 1947 Twins
Exc Zenker C-P
Exc, Formol- C-P Zenker
P
Transitional to next stage
Caudal neuropore widely open
Macerated. Upper limb buds not visible
No upper limb buds. Caudal neuropore closed
1924 Neural tube folded Al. coch. 1925
p 1924
1953



==STAGE 13==

Serial Size Embedding
No. (mm) Grade Fixative medium Plane
Thin- ness
(|xm) Stain Year
Notes
Obtained by Mall while student
Abnormal. Nasal discs fused
1 E.,4.5
Ch., 30X30
19 E., 5.5
Ch., 18X14
87 E., 4
Ch., 24X16x9
76 E., 4.5 Ch.,22X20
112 E., 4 116 E., 5 148 E.,4.3
Ch., 17x14x10 186 E.,3.5
Ch., 25X20X15 239 E., 3.0
248 E., 4.5
Ch., 30x23x15
407 E.,4
Ch., 14x13X7
463 E., 3.9
Ch., 17x12x7
523 E., 5
Ch., 25X25X15
588 E., 4.0
Ch., 19x15x8
786 E., 4.5
Ch., 19X10X10
800 E., 6.0 808 E.,4.0 826 E., 5.0
Ch., 13X13X9 836 E.,4.0
Ch., 22X18X11 963 E.,4.0
Ch., 23X18X16 1075 E.,6.0
Ch., 46x32x20 3956 E., 4.0
4046 E.,5
Ch., 22X20X20
5541 E., 6.0
Ch., 35x30x20
5682 E., 5.3
Ch., 29x25x13
5874 E., 4.8 6032 E., 5.8
Ch., 30x24x13 6469 E., 5.0
Ch., 25x18X18 6473 E., 5.0
Ch, 30X30X15 7433 E., 5.2
Ch., 15 x 1 3 x 1 3 "618 E,48
Ch, 18x15x15 "669 E,5.0
Ch., 23xl6x 14 "'889 E, 4.2
8066 E,53
C h , 20 x 18 x IS
Poor Salicylic acid Poor p Poor p Poor Ale.
P Trans. ? Trans.
10 20 20 20 10
p 10
20
10 50
40
10
p
15
15
10 15 20
15
20
20
20 50
10 20 10
? p 6
8 10 6
6 8
Hemat. 1887
Al. coch. 1895
Al. coch. 1896
Al. coch. 1897
Al. coch. p Al. coch. 1898 H.&E. 1899
Al. coch. 1901
H.&E. 1903 Al. coch. 1904
Al. coch. 1907
Al. coch. 1910
Al. coch. 1911
H.&E. 1912
Al. coch. 1913
H. 1913 Al. coch. 1914 Al. coch. 1914
Al. coch. 1914
Al. coch. 1914
H.&E., or. G. 1915
Al. coch. 1922 Al. coch. 1922
Al. coch., eosin 1927
Al. coch. 1928
H.&E. 1929 p 1929
p 1932
Al. coch. 1932
H.&E. 1937
H.&E. 1939
H.&E. 1939
H.&E 1941 H.&E 1942
Poor p Poor p Poor AJc.
Poor Ale.
Poor Formol Poor p
Poor Formol
Good Formol
Fair Formol
Good Corros.
Poor Ale.
Good Corros. Poor Corros. Good Formol
Exc. Corros.
Good Formol
Exc. Corros.
Poor Formol Poor Formol
Good Formol Poor Formol
Exc. Bouin Poor Formol
Poor Formol
Exc. Formol
Exc Formol
Exc. Bouin
Good Formol
Exc. Bouin Exc. Bouin
acetic
acetic acetic
acetic
acetic
p P
p
? P
P P
?
?
P p
P
P
P P P
P
P
P
Trans.
Trans.
Sag. Sag. Cor.
Trans.
Trans. Cor.
Trans.
Cor.
Trans.
Cor.
Sag.
Trans. Trans. Trans.
Trans.
Cor.
Cor.
Trans. Trans.
Trans. Cor. Trans.
p
p
Cor.
Cor.
Cor.
Cor.
Cor. Trans.
Advanced
Curettage. Anencephaly Tubal. Incomplete Shrunken
Less advanced
Most advanced in group Tubal. Incomplete
Hysterotomy. Bromides only Not good enough to cut
Fragmented on cutting. Not saved
Less advanced. Ag added
Tubal
Hystereaomy. Advanced. Ag added
Hysterectomy. Least advanced in group, Ag added
Hysterectomy
Hysterectomy. Ag added to slide 2
P P
P P P
OP
C-P
C-P
C-P
C-P C-P
p
p
Serial Size No. (mm)
8119 E., 5.3
Ch., 32x28x6.5
8147 E., 5.2
Ch., 27X21X19
8239 E., 4.3 8372 E., 5.6 8581 E., 4.8 8967 E., 5.7
9296 E,4.5
9297 E., 4.5
9697 E., 5.5
Grade Fixative
Exc. Bouin
Poor Formol
Exc. Bouin
Exc. AJc. & Bouin Good Kaiserling Exc. Acetic Zenker
Embedding medium
C-P ?
C-P P C-P C-P
C-P
Thin- ness Plane (|xm)
Trans. 8
?p
Sag. 8 Trans. 10 Sag. 8 Trans. 6
Cor. 8
Stain Year
H.&E. 1943
p 1943
H. phlox. 1944 Azan 1946 Azan 1948 H.&E. 1931
Azan 1955
Notes Hysterectomy
Tubal. Not cut
Most-advanced third
Head injured. Univ. Chicago No. H1426
Not cut
Exc. •> Exc. •>
STAGE 13 (continued)
Sag. 8
Bouin 1956
C-P
Azan 1955


==STAGE 14==


Serial No.
4 18
80
187
208
245
372 380
387 442 552 560 676 873 988
1380
1620
11%
2841 3360
Size (mm)
E.,7
E.,7
Ch., 18X18
E., 5.0
Ch., 24x18x8 E.,7
Ch., 35x30x25 E.,7
Ch., 22X11X11 E.,6
Ch., 13X12X10 E.,7
E,6
Ch., 20x20x14 E.,7
Ch., 45x40x50 E.,6
Ch.,25x20
E.,6
Ch, 40X28X28 E., 7.0
Ch, 24x24
E., 6.0
Ch, 35x20x17 E,6.0
Ch., 35x28x16 E,6.0
Ch., 38x30x23 E , 5."7
Ch, 36x24x24 E, 6.6
Ch, 35x30x8 E, 6.68
E , sy
Ch. 35x21
E.6.0
Grade
Poor p Poor p
Good Ale.
Poor ?
Poor ?
Fixative
Embedding medium Plane
Thin- ness (UJTI)
10 20
20 20 20
5
10 20
20 50 40 40 2 0 2 0 20 20 20
6 20
20
Stain Year
Al. coch. 1892 Al. coch. 1895
Al. coch. 1897
Al. coch. 1902
Al. coch. 1902
H.&E. 1904
H.-Congo red 1902 H.&E. 1906
H.&E. 1907 Al. coch. 1908 Al. coch. 1911 Al. coch. 1912 H.&E.
Notes
Poor Formol, Zenker
p
P Trans. •> Sag.
p Sag.
? Trans.
Fairp p Poorp p
Trans. Sag.
Good Formol Poor Formol Poor p
Poor Formol Good Carnoy Poor Formol
P Trans. p Cor.?
P Sag.
P Cor.
P Tr.-Cor.
P Sag.
P Trans.
P Cor.
P Sag.
? Trans. P Trans.
C Trans
Possible anencephaly
Good
Exc.
Good
Fair Good
Good
Formol-corros. acetic
Formol
Formol
? Ale.
Formol
Al. coch. 1914
Al. coch. 1916
Al. coch. 1916
Al. coch., or. G. 1919 H.&E,or.G 1920
H.&E,or.G 1920
In mvomatous uterus
Trans. Trans.
1913 Possible spina bifida Al. coch. 1914


Serial Size No. (mm)
STAGE 14 (continued)
Thin- Embedding ness
Grade Fixative medium Plane (|im) Stain Year
Notes
3805 E., 5.9 Exc. 3960 E., 5.5 Good 4154 E,6.8 Poor
Ch., 33X31X20
4245-6 E., 7.0 Good 4629 E., 6.5 Good
Ch.,32x23
4672 E,8.2 Good
Ch., 40X34X25
4805 E., 7.3 Good
Ch., 15X8X9
5437 E., 7.0 Good
5654 E., 5.0 Good Ch., 30X23X17
5787 E., 6.8 Good Ch., 32x30x23
6428 E., 7.0 Good Ch., 30X28X25
6500 E, 4.9* Good
6502 E., 6.7* Exc.
6503 E., 6.3* Exc.
6739 E.,8 Poor 6830 E,5.5 Exc.
Ch., 47X23X15
6848 E., 7.8 Good 7324 E, 6.6 Good
Ch., 17x13x10
7333 E, 6.3 Good
7394 E, 7.2 Exc. Ch., 45X20X20
7400 E, 6.3 Good Ch., 35X25x20
7522 E., 7.7 Good Ch., 33x16x16
7598 E., 7.0 Poor Ch., 30x30x25
7667 E., 5 Fair Ch., 16x14x12
7829 E., 7.0 Exc. 7870 E,7.2 Exc.
Ch., 25X20X 13
8141 E,7.3 Exc.
15 H.&E.
20 Al. coch.
Ch.,33x28 8306 E.5.3
Ch., 27 8308 E, 5.85
Ch., 27X18X 18 8314 E,8
Ch.,23x22 S35"7 E., 6.5
8552 E,6.5 8999 E,6
Exc. Bouin C-P
Exc. Formol & Bouin C-P
Exc. Formol C-P
Good Formol C-P Exc. Ale. & Bouin C-P Exc. Ale. & Bouin C-P
Ch.,16x15
9695 E,8.5 ?
Not cut
Bouin P Formol C-P Ale. C-P
Formol P Formol C-P
Formol p
Formol C-P
Formol C-P Formol p
Formol p
Formol C-P
Souza? C-P Souza? C-P
Souza? C-P Formol C-P Formol C-P
Formol C-P Formol C-P
Formol C-P Formol C-P
Formol C-P
Formol C-P
Ale. C-P
Formol p
Bouin C-P Bouin C-P
Formol C-P
Trans. Cor. Trans.
Trans. Sag.
Trans. Trans.
Trans. Trans.
Sag.
Cor.
Sag. Trans.
Cor. Sag. Cor.
Cor. Trans.
Trans. Trans.
Cor.
Trans.
Trans.
Trans.
Trans. Trans.
Cor,
Trans.
Sag.
Trans.
Sag Trans. Sag.
1921 Evans embryo No. 168. Serial bromides only
1922 Blood vessels naturally injected 8 H.&E. 1923
15 Al. coch.
10 H.&E. 1924
20 Al. coch.
10 H.&E.
8 H.&E.
10 Al. coch., eosin
1924 Advanced 1924 Tubal
1927 Advanced 1928 Less advanced
10 Al. coch. 1928
6, Al. coch. 10
10 Al. coch. 5,
1931 Advanced
1931 E. Leitz, Berlin
1931 E. Leitz, Berlin. Ag added to slides 1-25
10 H.&E.
10 Al. coch.
20 H.&E. 1933
1931 E. Leitz, Berlin 8 H.&E. 1933
10 H.&E. 8 H.&E.
1934 Tubal
1936 Low implantation
8 H.&E. 1936 8 H.&E. 1937
10 H.&E. 1937
8 H.&E. 10 H.&E.
1938 Natural blood injection
1938 Macerated 8 H.&E., phlox. 1939
8 H.&E. 8 H.&E.
8 H.&E.
10&20 H.&E., phlox. 1945
8 Azan 1945 8 Azan 1945
8 Azan 1946 8 Azan 1947 8 Azan 1952
1955
1923 Univ. Pennsylvania No. 40. Ag added
1940 Advanced
1941 On borderline of next stage. Ag added
1943 Shrinkage cracks in brain





==STAGE 15==


389 721
810
855 1006
1091 1354 1767 2743 3216 3385
3441
3512
3952
4602
4782
5772 5«92 f>223 65<W (rfOft
E., 9
E., 9.0
Ch., 30x20x10 E., 7.0
Ch, 30x25x15 E.,7.5
E,9.0
Ch., 37x26x22 E,7.2
Ch., 28x26x20 E,7.8
Ch, 35x30x25 E , 11.0
Ch, 41 X23X5 E., 7.2
Ch., 19xl8x 14 E, 6.5
Ch, 30x30x5 E,83
Ch., 25x20x16 E,8.0
Ch., 25x24x20 E,8,5
Ch., 33x28x25 E,6,7
Poor
Good Formol
Good Formol
Poor p
Exc. Zenker formol
Good Ale. Poor Formol
P P p
P P
P P
P P P P P P P P P P P P
Al. coch. 1914 20 H.&E., or. G. 1914
Ch., 30x25x15
E,9.3
Ch,, 33x30x26
E,9.0 Poor Ch., I4xl3x 11
10 Al.
10 Al.
15 Al.
15 Al.
20 Al.
coch. 1921
coch. 1921
coch. 1922
coch. 1924
coch. 1924
E, 8 Poor
E,-3 Good
E,f Poor Ale. E. "5* Exc.
F, "5* Exc.
E , "" V Good
E , HO Good
F , H4 Good
6595
"1W
-Mx 1" y5 Poor Formol
H.&E. 1932 Al. coch., phlox. 1935
Ch 35AMJx25 KV29 E , M 5
Ch, HH« 10 1 »9 *** F "'I
(h \"-29«i> mm I HH
jr Mil
I " f t
Exc. Ak. & Bouin C-P GlK»d Zenker C-P
Trans. 10 Sag. 10
Azan
1951
Poor Ale.
Poor Poor Good Good Poor Good Exc. Good Good Good Good
Formol Formol Formol Formol Formol Formol Corros. acetic Formol Formol Formol Formol Formol
Cor. Cor. Sag. Sag. Trans. Trans. Trans. Sag. Trans. Cor.
Sag. Cor.
Cor.
Cor.
Sag.
Sag.
Cor.
Trans. 6
Sag. 10
Cor. 10
Cor. 8 H.&E. 1936
p pp
?
Corros
15 Al. 10 Al.
coch., eosin 1928
Corros. Formol Formol
6 Al. coch. p 10 Al. coch. p Al. coch. p
acetic C-P C-P C-P C-P acetic C-P C-P C-P C-P
coch., phlox. 1929 8 Or.G. 1930
Exc. Zeriker
Exc Formal&Bouin p Cor.&Sag. 8 Azan 1952 Good Bouin p Cor. 8 Azan 1953
C-P
Trans.
Thin- Embedding ness
Serial Size
No. (mm) Grade Fixative medium Plane (|xm) Stain Year
Notes Least-advanced third
Shrunken and cracked Tubal
Median in group
Pathological between limbs Operative. Most-advanced third
Macerated
Least-advanced third
Most-advanced third
Macerated. Least-advanced third
Hysterectomy. Least-advanced third
Some sections lost. Most-advanced third. Ag added
Median in group Medical abortion
Transitional to next stage Fragmented sections. Not saved Least-advanced third. Ag added Most-advanced third. Ag added Many sections lost. Advanced
Operative. Good fixation Macerated. Median in group
Most-advanced third
Less advanced. Univ. Chicago No. H 1454
Univ. Chicago No. H 1324
Caudal quarter embedded separately Mechanically distorted
2 E., 7.0
Ch., 25X25X25
88 E.,8
Ch., 30x28x15
113 E.,8 241 E,6.0 371 E,6.6
Good Ale. P
Trans. 15 Cor. p
Sag. 10 Trans. 10 Sag. 10 Sag. 20 Trans. 15
Sag. 20 Trans. 100
Al. carm. 1888 Al. coch. 1897
Borax carmine ? H. & Congo red 1904 Al. coch. 1913 H.&E. 1907 H.&E. 1913
Al. coch 1913
20 Al. coch. 1915 20 Al. coch. 1916 40 H.&E., or. G. 1917 20 Al. coch. 1919 20 Al. coch. 1920 20 H.&E., or. G. 1921
H.&E.
6 H.&E. 1928
1932



==Stage 16==


Serial Size No. (mm)
163 E., 9.0
Ch., 35X35X20
221 E., 7.5
Ch., 40X33X33
383 E., 7.0
Ch., 15X15X15
397 E., 8.0
Ch., 15X15X15
422 E., 9.0
Ch., 30X30X30
559 E., 8.6
CH., 20X15X12
589 E., 11
Ch., 30X13X13
617 E., 7.0
Ch., 18X14x12
636 E., 10 Ch.,28x28X22
651f E., 7
Ch., 25X20X15
675 E., 10
Ch., 50X30X25
792 E., 8.0
Ch., 40X30X30
887 E,9.0
Ch., 31X28x17
1121 E., 11.8 1197 E., 10.0
Ch., 23X19X15 1544 E., 7.2
1836 E., 11.0 4677 E., 9.5
Ch., 48x36x30 5515 E., 12.0
Ch., 47X37X25 6054 E., 7,0
Ch., 21X17X12 6507 E., 9.0*
6509 E, 8.1*
6510 E., 10.1*
6511 E, 8.1*
6512 E., 7.0*
6513 E., 7.2*
6514 E., 9 0*
6516 E., 10 5*
6517 E., 10.5*
6686 E., 11.0
Ch., l7x 17XP 6750 E., 10.0
6909 E., 11.0 6931 E., 8.8
Ch., 3""x 33x16 6950 E.. 9 0
Ch., 3lx20x 18 "M15 E., 9.7
Embedding Grade Fixative medium
Good Formol P
Poor Formol P
Poor Formol P
Poor Formol P
Poor Ale. P
Good Formol P
Poor p P
Good Formol P
Poor Formol P
Poor p p
Poor Formol P
Good Formol P
Good Formol P
Good Corros. acetic P Good Formol C
Good Zenker P Good Formol P Good Formol P
Good Formol C-P Good Formol C-P
Exc. Corros. acetic C-P Exc. Corros. acetic C-P Exc. Corros. acetic C-P Good Corros. acetic C-P
Exc. Corros. acetic C-P Good Corros. acetic C-P
Pcx)r Corros. acetic C-P Good Corros acetic C-P
Thin- ness Plane (jxm)
Trans. 20 Sag. 20 Trans. 10 Trans. 10 Trans. 40 Trans. 20 Sag. 50 Trans. 15 Trans. 50
p p
Sag. 100
Trans. 20
Trans. 40
Cor. 40 Sag. 20
Sag. 20 Trans. 20 Trans. 10
Trans. 10 Trans. 8
Cor. 10&8 Cor. 10 Cor. 10 Sag. 10
Trans. 10 Cor. 10
Sag. 10 Sag. 8
Stain Year Al. coch. 1899
Al. coch. 1903
Al. carm., H. & 1904 Congo red
H.&E. 1907
Al. coch. 1910 H. & Congo red 1911 Al. coch. 1912 Al. coch. 1912 Al. coch. 1913
p 1913
Carmine 1915
Al. coch. 1913
Al. coch. 1914
Al. coch. 1915 H.&E., or. G. 1915
Al. coch. 1916 H.&E. 1917 Al. coch. 1924
H.&E. 1927 H.&E. 1930
Notes Used by Bardeen and Lewis
Macerated
Tubal. Partly macerated
Cyclopia. Formerly listed as stage 17
Median in group Macerated
Spina bifida
Abnormal head and limbs Advanced
Near next stage
Operative. Median in group Advanced
Tubal. Mechanical injury Most-advanced third Median in group
Near next stage
Least-advanced third
Ch. 30*23* 15
(continued)
Exc. Corros. acetic C-P Poor Formol
Good Formol Good Bouin Good Formol
Good Formol
Exc Bouin C-P
STAGE 16
Trans. 8 C-P Cor. 20
Close to No. 6507. Ag added
Surface injured by fixative. Most-advanced third
Least-advanced third. Borderline
Surface injured by fixative. Least advanced in group
Most-advanced third
Most-advanced third. Double left kidney and ureter
Close to No. 6516
Tubal. Partly macerated
Tubal. Advanced
Tubal. Advanced
Least-advanced third. Type specimen
Tubal. Partly fragmented Operative. Less advanced
OP Trans. 10 C-P Cor. 10 C-P Cor. 10
Cor. 10
C-P Trans 10
Al. coch. p Al. coch.
Al. coch.
Al. coch., iron H. •>
Al. coch. p Al. coch p
Al. coch.
Al. coch. >
Al. coch. ? Al. coch. 1933
H. & phlox. 1933 H.&E. 1934 Al coch., phlox. 1934
H.&E. 1934 H. & phlox 1935
Middle or most-advanced third p Least-adianced or middle third





Serial No.
7629
7804
7897
8098
8112 8179
8436 8692
8697 8773 8971
9055 9229
Size (mrn)
E., 11.5
Ch., 31X31
E., 9.5
Ch., 26X21X16 E., 12.2
Ch., 31x24x23 E., 10.0
Ch., 30
E., 10.9
E., 11.9
Ch., 23X18X17 E., 10.9
Ch., 13x15x1? E., 10
E., 11.3
E, 11
E., 10
Ch., 20.5x14.5x13.7 E., ca. 10
E, 9.5
Embedding m e d i u m
C-P
C-P
C-P
C-P
GP C-P
P P
Plane
Cor.
Trans.
Trans.
Cor.
Cor. Cor.
Cor. Trans. Trans.
Thin- ness
(M-m) S t a i n
10 Al. coch., phlox.
10 H.&E.
10 H.&E.
6 H.&E.
8 H.&E. 10 H.&E.
10 Azan
10 H.&E.
10 H.&E. 10 Azan 15 H.&E.
20 Azan & Ag 6 Ag & H.&E.
Y e a r
1939
1940
1941
1942
1943 1943
1946 1949
1949 1950 1932
1953 1954
Notes
Hysterectomy. Most advanced in group
Least-advanced third
Tubal. Advanced
Tubal. Median in group
Most-advanced third Tubal
Advanced
Rubella. Medical abortion. Mechanically damaged
Perhaps stage 17
Synophthalmia. Univ. Chicago No. H 1439
Damaged
Stage 15, 16, or 17? Mislaid
Grade Fixative
Good Formol
Good Formol
Good Formol
Good Formol
Exc Bouin Good Formol
Good Formol Good Bouin
Poor Formol Exc. Bouin Poor Formol
Exc. Bouin P Exc. Formol P
STAGE 16 (continued)
C-P
P Cor.
Trans.
Trans. Trans.



==Stage 17==

Serial Size
No (mm) Grade
353 E, 11.0 Good Ch., 40x35x20
485 E, 130 Exc. Ch, 33x25
544 E, 11.5 Good Ch., 30
562 E, 130 Poor Ch., 28x17x17
623 E., 10.1 Good
695 E, 135 Poor Ch., 40x40x17
916 E, 110 Good Ch, 30x30x16
940 E, 14.0 Good Ch, 28x23x21
1232 E, 14.5 Poor Ch., 35x35x30
1267A E., 14.5 Good Ch., 35x30x26
Fixative Formol
Formol Zenker-formol Formol
Ale.
Formol
Bouin
Formol
Formol
Formol
STAGE 17
Thin- Embedding ness
medium Plane (u.m) P Cor. 10
P Cor. 40 P Sag. 40 P Sag. 100 P Trans. 20 P Trans. 10 C Trans. 40 C Trans. 40 P Cor.
Stain H.&E.
Al. coch.
Al. coch.
Al. coch.
H. & Congo red H. & Congo red H.&E, or. G. H.&E, or. G.
Al. coch. H.&E., or. G
H.&E. Al. coch.
Al. coch. H.&E. Al. coch.
Y ear 1906
1911
1911
1912
1912
1913
1915
1914
1915
1915
1917 1928
1929 1930 ?
Notes Very advanced
Injected (India ink)
Operative. Injected (India ink) Advanced
Operative. Median in
group Macerated
Most-advanced third Advanced
Close to No. 1267A Excellent C.N.S. Tubal
Right upper limb injured
Most advanced in
group
Median in group
Least-advanced or middle third
(continued)
V]
5642
5893 62SH
6519
E, 12,5 Good E, 11.5 Good Ch.. 33x30x17
E.. 13.2 Good
E. 14 0 Good Ch . 48 x 35 x 25
E.. 108* Exc
Formol p
Formol Formol Formol Corrov acetic
c Sag. 20 40 Sag. 20 p Trans. 15
C-P Trans. 20 C-P Trans. 10 C-P Sag. 8
40





Thin- Serial Size Embedding ness
No. (mm) Grade Fixative mediurrI Plane (fxm) Stain Year
Notes
Median in group. Ag added to slides 1-25 Sections vary in thinness
Tubal. Advanced
Good primary germ cells
Least-advanced third
His embryo "Ru." Every third section Most-advanced third Operative
Middle third
Operative. Least advanced in group
Univ. Chicago No. H566
Univ. Chicago No. H1475
Mislaid
6520 E., 14.2* Exc.
6521 E., 13.2* Exc.
6631 E., 13.0 Good 6742 E., 11.0 Good Ch.,50X40X15
6758 E., 12.8 Good 7317 E., 10.0* Good
7436 E., 13.0 Good 8101 E., 13.0 Exc. 8118 E., 12.6 Exc. 8253 E., 11.2 Good
Ch.,30X20X10
8789 E., 11.7 Exc. 8969 E., 11.2 Exc.
8998 E., 11.0 Exc. 9100 E., 12.0 Exc.
Ch., 12X13X10
9282 E., 12.0 Good
Ch., 16
Corros. acetic
Corros. acetic
Formol Formol
Formol
Formol Bouin Bouin Bouin
Bouin
7 7
Formol-chrom. subl. Ale.
C-P Trans. 10
C-P Trans. 8-18
C-P Cor. 10 C-P Trans. 12
C-P Trans. 10 P Cor. 10
C-P Cor. 30 C-P Trans. 10 C-P Cor. 10 C-P Cor. 10
C-P Sag. 10 p Trans. 15
C-P Cor. 10 C-P Sag. 10
p Trans. 15
Al. coch. p
Al. coch. ?
H.&E. 1932 H. & phlox. 1933
H. & phlox. 1933 H.&E. 1936
Al. coch. 1937 H.&E. 1943 H.&E. 1943 Al. coch., phlox. 1944
Azan 1950 Azan 1919
Azan 1952 Azan 1933
Ag 1955

==Stage 18==

Serial Size
No. (mm) Grade
{|
|-
| 109 || E., 12.0* Poor Ch.,30
|-
| [[:Category:Carnegie Embryo 144|144]] || E., 16.0* Good Ch, 40x30x30
|-
| I?? || E., 13.0 Ch, 30x25x25 || Poor 
|-
| 296 || E., 17.0 || Poor
|-
| 317 E., 16.0 Good 
|-
| 351 E., 14.0* Good
|-
| 406 E., 16.0 Good Ch., 40x40x40
|}


Thin- Embedding ness
Fixative medium Plane ((J-m) Ale. P Trans. 20 Formol P Sag. 40 Ale. P Trans. 20 Ale P Cor. 20
Stain Al. coch. Al. eoch. Al. coch.
P.-M. Semic. duct
ducts ( mm) Year
1 0.4 1897
3 0.85 1899
2 0.6 1900
Notes
Tubal. Least-advanced third
Most-advanced third Tubal. Partly macerated
Most-advanced third Middle third
Injected (Berlin blue)
Operative. Most-advanced third
Double infection. Advanced Injected (India ink)
Head injured. Most ad- vanced in group
Tubal Advanced
Median in group
Median in group
Operative. Head cut sepa- rately
Tubal Head injured
Advanced
423 E., 152
424 E., 17.2
492 E, 16.8
Ch, 40 x 40 511 E., 160*
Ch., 3«* 32x32 670 E, 12.5
"?19 E, 15 0
Ch, 50x50x50
733 E., ISO
Ch., 4Sx40x2S
841 F... 15.0
Ch., 18 x 16x9
899 E, 16 0*
Ch. 50 x 18 x IS
Good Formol-Zenker P Good Formol P Exc. Zenker P
Good Ale P
Poor Ale. P Gcxxi Formol P
Ptx>r F(>rmol P Good Formol P G(Kxi Bouin P
Carmine Carmine Al. coch.
Al. coch.
H.&E. Al. coch.
Al. coch.
H.&E.. car- mine
Al. coch.
3 0.85 1904 3 1.0 1904 3 0.7 1911
3 1.1 1911
3 1.0 1913 2 0.6 1913
2 0,6 1913 2 032 1914 3 0,65 1914
991 E. l"0 Good
Formal P
Sag so R, V, Gieson 3 0.9 1914
Formol P Formol P
Formol P
Cor. 20 Cor. 250
Sag. 20
Trans. 50 Trans. 50 Cor. 40
Sag. 40
Sag. 50 Trans. 40
Sag. SO
Cor.& 10 Trans, 20 Sag. SO
3 0.85 1905
0.7 1905 038 1904
0.7 1907

Various
H.&or.G. 2 Slightly car- 2 mine
H.&E. 3






Serial Size
No. (mm) Grade
1909 E., 14.6 Good 2673 E., 15.5 Good 4430 E., 14.0 Exc.
Ch, 51x40x21
5542B E., 16.0 Good Ch, 37X32X25
5747 E, 15.2 Poor Ch, 32X27X25
5935A E, 13.5 Good Ch, 40 x 30 x 30
6522 E , 13.2* Good
6524 E,11.7* Exc.
6525 E , 13.8* Exc.
6527 E , 14.4* Exc.
6528 E, 13.4* Exc.
6529 E , 15.6* Good
6533 E , 12.5* Good 6551 E,18.0 Poor 7707 E,14.5 Exc.
Ch,37 x 32
8097 E,15.5 Good
Ch, 37x25x21
8172 E, 16.5 Exc.
8235 E, 14.0* Good
8355 E, 15.0 Exc. Ch, 23
8812 E, 12.9 Exc.
8945 E, 13.9 Good 9107 E, 17.0 Good Ch, 38 x 28 x 22
9247 E, 15.0 Exc.
Fixative
Formol Formol Corros. acetic
Formol
Alc.-formol
Thin- Embedding ness medium Plane (jjum)
Stain
Semic. ducts
P.-M. duct ( m m )
0.3 0.52 0.9
Y ear Notes
1917 Less advanced
1919 Median in group 1923 Most-advanced third
1927 Other twin abnormal
1928 Least-advanced or middle third
Other twin stunted
Middle or most-advanced third
Least-advanced third
Weak staining
Mechanical damage Least-advanced third Middle third
Middle third
Tubal
Operative. Middle third
Least advanced in group
Operative. Very advanced Tubal
Formol
Corros.
Corros. Corros. Corros. Corros. Corros. Corros. Formol Bouin
Formol
Bouin Bouin
Formol
Formol
Zenker Bouin
acetic
acetic acetic acetic acetic acetic acetic
Al. coch.
Al. coch.
Al. coch.
Al. coch.
Al. coch.
Al. coch.
H.&E. 3 0.8 1932 H.&E, phlox. 2 0.54 1939
H.&E. 1 0.19 1942 H.&E. 3 0.58 1943
Bouin
C-P Sag.
8
Azan
1954 Tubal
STAGE 18 (continued)
P Cor.
P Trans. P Trans.
P Trans.
P Sag.
P Cor.
C-P Cor.
C-P Trans. C-P Sag. C-P Trans. C-P Cor. C-P Cor. C-P Sag.
p Cor. C-P Trans.
C-P Trans.
C-P Trans. C-P Sag.
C-P Cor.
C-P Trans.
p Trans. p Trans.
20 40 15
40 25 40 10 10
8 15
8 10 6, 8, 10 40 10
10
20 10
10 10
8 15
Al. coch, Al. coch. Al. coch,
Al. coch.
Al. coch.
Al. coch.
Al. coch.
or.G. 1 2
or. G.
H.&E., Mal- lory
Azan
H.&E.
Borax, carm. Borax, carm.
2 0.25 1944
3
2
2
1 0.38 1929 3 0.8 ? 1 0.4
2 0.42 2 0.67 1 0.33 2 0.4 2 0.45
0.7 0.25
1946 Tubal. Duplicated spinal cord caudally
1950 Rubella. Medical abortion. Midbrain punctured
1952 Univ. Chicago No. H 1254 1918 Univ. Chicago No. H 516

==Stage 19==

{| class="wikitable"
|-
! Serial No. !! Size (mm) !! Grade !! Fixative !! Embedding Medium !! Plane !! Thinness (µm) !! Stain !! Point Score !! Sex !! Year !! Notes
|-
| 17 || E,18 Ch, 40x30x20 || Good || Alc.|| 
| 43  ||  Poor || Alc. ||
|-
| 293  || Good || Formol? ||
|-
| 390 || Good Formol  || 
|-
| 409  || Good Formol  || 
|-
| 432  || Good Formol || 
|-
| 576  ||  Good Formol  || 
|-
| 626  || Poor ||  Formol  || 
|-
| 6?8  || Poor || Alc || 
|-
| 709  || Good || Formol || 
|-
| 837 || || Al. coch. || 
|}






E, 16 E, 19 E, 19
E. 18
Ch, 50x40x40 E.. 18.5
C h , 45 x 35 x 20 E. 17
Ch, 60x40
E., 21.5
Ch., 40x30x21 E, 20
Ch, ca. 30
E, 19
Ch. 40x35x25 E. 21
Ch. 65X45x -W
50,
100
50
Sag. 50 Coch.
Grade
Poor Ale.
Fixative
Stain Al. carm.
Year Notes 16.5 9 1894
10 9 1894 16.5 S 1905
11.5 9
Sag. Trans. Sag. Sag. Trans. Sag. Cor Sag.
20, H.&E. 50
1906 Tubal. Injected 9 1907



20 Copper, iron H. & 14.5 erythrosin
20 H. & Congo red 13.5
15, H.&E. 14.5 20
100 Al. coch. 14.5 50 Al. coch 12 40 Al. coch, Lyons blue 15 40 Al. coch. 14.5
9
d
6 1913
9
9 1913
1910 Tubal 1912 Tubal
1913 Head damaged
1914




279
Serial Size No. (mm)
|-
| 1324 E., 18 Ch., 50x30x18
|-
| 1332 E., 19 Ch., 40x43x22
|-
| 1390 E., 18 Ch, 40X38X15
|-
| 1584 E, 18 Ch., 35X31 X 25
|-
| 2114 E., 19.3 Ch., 49x42x33
|-
| 4405 E., 15.5 
|-
| 4501 E, 18 
|-
| 5609 E., 18 
|-
| 6150 E., 17 Ch., 40x39x30 
|-
| 6824 E., 18.5 Ch., 45x40x25 
|-
| 7900 E., 16.5
|-
| 8092 E., 16.3 Ch., 52 x 47
|-
| 8913 E., ? Ch, 34
|-
| 8965 E, 19.1 Ch, 42x32x19
|-
| 9097 E, 21 
|-
| 9113 E, 18.5 Ch, 24 
|-
| 9325 E, 17.0 Ch, 32x28x20



C Cor.
C Cor.
P Sag.
P Sag.
P Trans.
P Trans. P Trans. P Cor. C-P Trans.
C-P Sag.
C-P Sag. C-P Trans.
Exc. Formol-glucose C-P
Exc. Formol C-P
Good Formol-acetic p Trans.
Point
score Sex Year
12.5 ?9 1915
15 9 1915
10.5 9 1916
13.5 6 1916
12 9 1918
13.5 <3 1923 14 6 1924 13.5 6 \L)r 165 9 1930
Grade Fixative Good Formol
Poor Formol
Good Formol
Good Formol
Good Formol
Good Formol Exc. Bouin Exc. Formol Good Bouin
Good Formol
Good Bouin Exc. Bouin
Poor Formol
Good Formol-Zenker C-P
(|xm) Stain
40 H.&E. aur, or. G.
40 H.&E. aur, or. G. 20 Al. coch.
50 Al. coch.
40 Al. coch.
10 Coch, Mallory 15 Coch, or. G. 25 Al. coch.
15 H.&E.
12 H.&E.
20 H.&E, phlox. 20 H.&E., phlox.
Notes
p Trans. Trans.
10 Azan ?V 1951 10 Borax, carm, or. G. •> ? 1952
10 Azan ?? 1930 10 Azan > 6 1953
15& Azan & Ag •> 1955 8-10
Cor. Trans.
14.5 6
115 1941
Tubal
Protruding midbratn
Midbrain injured Cystic left kidney
Tubal
Tubal
Rubella. Medical abortion. Isolated head damaged
Univ. Chicago No. H 173
Univ. Chicago No H 1380 Rubella. Medical abortion
Tubal
1933 139 1942
Tubal

==Stage 20==


Serial Size No. (mm)
240 E, 20
Ch, 50x40x30
256 E,21 368 E, 20 431 E,19
Ch, 30 x 25 x 25 437 E, 23
Ch., 80X60X50 453 E, 23
Ch, 60X40X30 460 E, 21
462 E, 20
Ch, 50x40x30
635B E, 22
Thinness Plane (u.m)
Cor. 20 Sag. 25
Sag. 20 Sag. 20
Sag. 50 Sag. 20
Trans. 40 Trans. 40
Trans 50 Sag 40
Stain Iron H.
Coch.
H. & Congo red H. & Congo red
27 d
23 9 22,5 9 25,5 9
19W
1904 Tubal. 1906
190
657
966 1108
E, 25
Ch, 35x20x15 E, 23
Ch, 51X38X13 E, 19.8
Ch, 3OX25X 18 E , 21.3
Exc Bichlor. acetic P
26,5 9 25 9 2" 9
1134E E, 23
1266 E, 23.1
2393 Ch, 61.5x50x35
Pi >or
Formol p Formol C-P
20S 6
191*
Embedding Grade Fixative medium
Poor Formol
Poor Ale. P P o o r Ale. P Good Formol P
Poor Formol P Poor Formol P
Exc. Bichlor. acetic P Exc. Formol P
Poor Ale, P Poor Formol C
Cor 40 Poor Bichlor c Trans
Point
score Sex Year
Good Fc>rmol p
Sag, 100 Sag 100 Sag, 25
STAGE 20
Coch 24 H. & Congo red 23-5
24,5 9 23.5 6
Al. coch
H.&E, aur, or. G
Al. coch.
H.&E aur, or G 21,5 Al. coch, 22
1910 tojectcd 1910
1913 191.
1914
H.&E., coch, Al, coch
Al cwh
H&E aur,ur G 2*5


Serial Size No. (mm)
3527 E., 22
Ch., 32X30X10
4059 E., 21.6 4148 E, 21
Ch., 45X34X30 4361 E.,22
Ch., 52x42x23 5537 E.,22
6202 E, 21
Ch., 35X35X22
6426 E, 21.5 7274 E, 18.5
Ch., 48x44x35 7906 E, 19.5
8157 E., 20.8 8226 E, 18.0
Grade Fixative
Good Formol
Good Formol Good Formol
Poor Formol
Embedding medium
Thinness Plane (|xm)
Stain Al. coch.
Al. coch., Mallory Al. coch., Mallory
Coch.
Al. coch. H.&E.
H.&E.
H.&E., phlox.
Point
score Sex Year
28? 1921 29.5 $ 1922
19 ? 1922 24 8 1923
21 8 1927 20.5 8 1930
21 8 1931 20 9 1936
22 8 1941 24 8 1943
8 1944
Notes
Exc. Bouin p Exc. Bouin p
Good Formol C-P Exc. Bouin C-P
Exc. Bouin C-P Exc. Bouin C-P Exc. Bouin C-P
Trans. 20 Trans. 20
20
20 20
Tubal
STAGE 20 (continued)
Sag.
Cor. Cor.
25
15 15
Trans. Trans.
Cor. 20 Cor. 20 Sag. 10
H.&E.
H.&E.
Azan ?
Left renal agenesis



==Stage 21==
{| class="wikitable"
|-
! Serial No. !! Size (mm) !! Grade !! Fixative !! Embedding Medium !! Plane !! Thinness (µm) !! Stain !! Point Score !! Sex !! Year !! Notes
|-
| 22 || E, 20  Ch, 35x30x30 || Good || Alc. || P || Trans. || 50 || Al. coch. || 34.5 || F || 1895 ||
|-
| 57 || E, 23 Ch., ca. 30 || Poor || Alc. || P || Sag.||  50 || Al. coch. || 36 || M || 1896 ||
|-
| 128  || E, 20 Ch., 50x43 || Good  || Formol || P || Cor. || 50 || Al. coch. || 33 || F || 1898 ||
|-
| 229  || E, 19  || Poor  || Alc. || P || Sag. || 50 || Al. coch. || 33 || F || 1903 || 
|-
| 349 || E, 24  || Good || Zenker || C || Cor. || 250 || Unstained || 36 || ? || 1905 || Double vascular injection
|-
| 455  || E, 24 Ch., 42x34x20 || Good || Alc. || P || Trans. || 30 || {{HE}} || 36.5 || M || 1910 ||  
|-
| 632  || E, 24 Ch., 60x50x30 || Good || Bichlor. acetic || P || Sag.|| 40, 100, 250 || Al. coch. || 33 || F  || 1913 || Injected
|-
| 9O3C  || E, 23.5  || Good  ||  Formol || P || Trans. || 40 || Al. coch. || 38.5 || F || 1914 ||
|-
| 1008  || E, 26,4  || Good  ||  Formol || P || Sag.|| 40 || Al. coch. || 39 ||  ?? || 1914 ||
|-
| 1358F || E, 23 || Good  ||  Formol || P || Sag.|| 40 || Al. coch. || 37.5 ||| F || 1916 || 
|-
| 2937 || E,, 24.2  || Good  || Bouin || P || Trans. || 50 || {{HE}} aur., or. G. || 39 || F || 1920 ||
|- 
| 3167 || E., 24.5 Ch., 60x50x40  || Poor || Bichlor, acetic, formol ||  P || Trans. ||  20 || Al. coch. || 32 || M || 1920 ||
|-
| 4090 ||  E, 22.2 Ch.. 66x46x30 || Good  ||  Formol || P || Trans. || 40 || Al. coch. || 30 || F || 1922 ||
|-
| 4160  || E,25  || Poor ||  Formol || P || Sag.|| 25 || {{HE}} || 39 || M || 1923 || Tubal
|-
| 4960  || E.22 Ch,, 47x42x28 || Good || Formol || P ||  Trans. || 15 || Al. coch., Mallory || 31.5 || F || 1925 ||
|-
| 5??6  || E. 215  || Good || Formol || P || Sag.|| 20 || {{HE}} || 34 || F || 1927 || 
|-
| 6531  || E,22  || Poor  || Glacial acetic, || C-P || Trans. || 10 || {{HE}} || 31.5 || F || 1931 || Leitz Collection ||
|-
| 72S4  || E,225  || Exc || Bouin || C-P || Trans. || 20 || {{HE}} || 33.5 || M || 1936 ||
|-
| 7592 || E,22-> || Exc. || Bouin || C-P || Trans. || 20 || {{HE}} || 36 || F || 1937 || 
|-
| 1*64  || E., 24  || Exc,  ||  Formol || C-P || Frontal || 20 || {{HE}} || 32.5 || M || 1941 ||
|-
| 8553  || E., 22  || Exc  || Bouin ||  C-P || Trans. || 12 || {{HE}} || 38 || F || 1947 ||
|-
| 9614  || E,,22 5  || Exc  || Bouin || P || Cor. || 10 &15 || Azan || ? || ? || 1958 || Rubella. Hysterectomy
|}



Year Notes 34.5 8 1895
36 6 1896 33 6 1898 33 9 1903


Formol
Ale. Zenker Ale.
Bichlor. acetic
Formol Formol Formol
Bouin
Bichlor, acetic, formol
Formol
Formol Formol
Formol
Glacial acetic,
¥*t!*£?• «1L,
Bouin C-P Bouin C-P Formol C-P Bouin C-P Bouin p
36
36.5 6 1910
P
P
P
P C P
P
P P P P P
P
P P
Trans. 50
Sag. 50
Cor. 50
Sag. 50 Cor. 250 Trans. 30
Sag. 40, 100,
Al. carm.
Al. coch.
Al. coch.
Al. coch. Unstained H.&E.
Al. coch.
Al. coch. 38.5
Al. coch. 39 Al. coch. 37.5 H.&E. aur., or. G. 39 Al. coch. 32
P C-P
H.&E.
H.&E. H.&E. H.&E. H.&E. Azan
31.5 9
33.5 6 1936
STAGE 21
Embedding Thinness Point Grade Fixative medium Plane (ixm) Stain score Sex
250 40 40 40 50 20
40
25 15
50 10
Trans.
Trans.
Frontal 20 Trans, 12 Cor. 10&15
1913 Injected 9 1914
a 1914 9 1916 9 1920 6 1920
Trans. Sag. Sag. Trans. Trans.
Trans.
Sag. Trans.
Sag. Trans.
Al. coch.
30 9 1922
20 20
1931 Leitz Collection 9 1937
39 6
9 1925
Al. coch, 34
H.&E.
Al. coch., Mallory 31.5
1923 Tubal 9 1927
33 9
36
32.5 6 1941 38 9 1947
? <J
1905 Double vascular injection
1958 Rubella. Hysterectomy

==Stage 22==

{| class="wikitable"
|-
! Serial No. !! Size (mm) !! Grade !! Fixative !! Embedding Medium !! Plane !! Thinness (µm) !! Stain !! Point Score !! Sex !! Year !! Notes
|-
| 392 || E., 23 Ch., 45x45x25 || Poor 
|-
| 405 || E., 26 || 
|-
| 584A || E.,25 Ch., 50x42x40 ||
|-
| 630 || E., 25  || 
|-
| 840 || E, 24.8  || 
|-
| 875 || E, 27 || 
|-
| 895 || E., 26 || 
|-
| 1315 || E.,25 || 
|-
| 1458 || E., 27.5 || 
|-
| 1894 || E, 24.6  || 
|-
| 2206 || E, 27 || 
|-
| 3681 || E, 26.3 || 
|-
| 4304  || E, 25 Ch, 66x45x45  || 
|-
| 4339  || E, 24.5 || 
|-
| 4638  || E, 23.4  || 
|-
| 6701 || E,24  || 
|-
| 6832  || E, 25.8  || 
|-
| 8394  || E, 25.3 Ch, 48x50x34  || 
|-
| 8948  || E, 26.7 || 
|}


P

42 9 1907
Notes
Brodel Collection. Injected
405 E., 26
4476 E., 26.2 


Ch., 40X28X22 
Ch., 67x62x54

Ch, 45X45X30

Ch, 50X30X18 
Ch, 36x36x34 




Carmine 42.5 <J 1907 Al. coch. 44.5 d 1910
Al. coch. 41 1913
464 E.,26
Good Formol C
Good Formol?, P Ch., 45X40X30 ale?
Ch, 61X51X50
Poor Formol
Poor Formol Good Formol Good Formol
Good Formol
Good Formol Exc. Formol
Good Formol Poor Formol
Good Formol Good Bouin
Good Formol Good Bouin Exc. Bouin Poor Formol Exc. Bouin Exc. Bouin
Poor Formol-Zenker
P
P P P
P
P C
c
p
p
p
Al. coch. Al. coch. Al. coch.
Al. coch.
Al. coch.
H.&E. aur, or. G.
H.&E. aur, or. G. H.&E.
Al. coch. H.&E.
Al. coch, Mallory H.&E.
Al. coch, or. G. H.&E.
H.&E.
H.&E, Masson
46 6 1913 44.5 9 1914 45 6 1914
46.5 9 1914
p
p
p
p
C-P Cor. 20 C-P T rans. 20
1923 1923
STAGE 22
Sag. 50
Sag. 40 Sag. 100
Sag. 50
Trans. 100 Trans. 50 Sag. 40
Trans. 25
Sag. 50 Sag. 50
Sag. 40,80 Trans. 40
Trans. 25 Trans. 20
Trans. 15 Trans. 40 Trans. 15,20 Cor. 20
Al. coch.
> Trans. 15Agp7 1952
40.5 9
45.5 6 1916
Spina bifida and anencephaly
1915 41 9 1917
44.5 6 1918 44.5 6 1921
44.5 9
46.5 9 1923
Injected Tubal
46 9
41.5 S 1924 41 9 1933 42 9 1934 44.5 9 1946

==Stage 23==

Serial Size Embedding No. (mm) Grade Fixative medium
45 E,28 Poor P
Ch, 40x35x20 trans.
Point
score Sex Year
515 9 1895 57 6 1897
•> 6 1897 57.5 7 1897 52.5 6 1897 54 6 1903
58.5 9 1907
56 6 1913
53 8 1913
75 E,30 Good Ale. P
86 E,30 Good 7 100 E,27 Poor 7P
Sag. 50 Cor. 50 Sag. 50
May be an early fetus
108 E, 28(est.) Poor 22^ E,30 Pcxtr
Ch, 60x40x20
417 E,32 Good
Ch., "^0x60x40
756A E, 2~7 Good
Ch..60x45x35
^82 E,28 Good
Ch, 80x80 x^O
950 E,29 Good
1199 E.,26 Good Ch,, 60x40x30
1535 E,28 Poor Ch. SOx45x 15
Picrosulph. acid Formol
Formol
Formol
Formol
Formol Formol
Formol
P Sag. 45 P Sag. 50, 100
P Trans. 100
P Cor. 50
P Trans. 40
STAGE 23
Thinness Plane (ujn)
Stain Al. coch.
Coch.
Coch.
Al. coch. Borax carm. Al. coch.
Al. coch. Al. coch. Multiple Al. coch.
Notes
Cor. 50
P Trans 50
C Cor 40 H.&E.aur,or.G. 54.5
P Trans. 40 H.&E 495 6 1916
54 6 1914 6 1915



Serial Size
No. (mm) Grade
Fixative Formol
Formol Bouin Formol
Formol Bouin
Bouin Formol Formol
Embedding Thinness medium Plane ((xm)
C-P Trans. 50
C-P Trans. 25 P Trans. 50 P Trans. 15,20
P Sag. 20 P Trans. 15
P Trans. 20 P Sag. 40 P Trans. 20
P Cor. 25 C Trans. 20 C-P Cor. 20 C-P Trans. 12
Stain H.&E. aur., or. G.
H.&E. aur., or. G. Al. coch.
Al. coch., Mallory
H.&E.
H.&E., phlox.
H.&E. H.&E. H.&E.
H.&E. aur., or. G. H.&E.
H.&E.
Azan
Ag
Point
score Sex Year
48 9 1917 48.5 3 1919
55.5 9 1923 59 9 1923
57 s 1924 55 6 1924
59.5 S 1926 52.5 9 1927 52.5 6 1927
50.5 9 1928 58.5 9 1932 47 9 1937
•> 3 1954 •? •> 1976
Notes
1945 E., 27.3
Ch., 83X53X22.5
2561 E., 27.5 4205 E., 29.5 4289 E., 32.2
Ch., 52X35X25 4525 E.,30
4570 E, 30.7
Ch., 52X50X28
5154 E.,32 5422 E., 27 5621A E., 27.5
5725 E., 23 6573 E., 31.5 7425 E., 27 9226 E., 31 D.122 E, 27
Good
Good Good Good
Good Exc.
Good Good Good
Good Good Exc. Exc. Exc.
Formol
Bouin
Bouin
Formol
-/ Trans. 19
Other twin has spina bifida and fused kidneys
Ag added
Yntema and Truex