Talk:Book - Sex and internal secretions (1961) 14

I. Introduction 797

II. Methods 798

A. Methods for Recovering Mammalian Eggs and Embryos 798

1. Collecting ova from the oviducts. . 798

2. Collecting free ova from the uterus 798

3. Recovery of attached embryos. . . . 799

B. Egg Culture and Preservation in Vitro 799

C. Intraspecific Egg Transfer 800

D. The Production of Eggs by Superovulation " 801

III. Biology of the Mammalian Egg 802

A. Oogenesis 802

B. Growth, Composition, and Size of the Mammalian Egg 807

C. Egg Membranes 811

1. The zona pellucida 811

2. The mucous or "albuminous" layer 815

D. The First Maturation Division ...... 81G

E. The Ovulated Egg 817

F. Respiratorv Activity of Mammalian Eggs. . .". : 818

G. Transport of Tubal Ova 819

IV. Fertilization and Implantation 827

A. The Cumulus Oophorus and Sperm Penetration 828

B. The Zona Pellucida and Sperm Penetration 832

C. Sperm-Egg Interacting Substances.. 834

D. Sperm Penetration of the Vitelline Membrane 834

E. Fertilization in Vitro 835

F. Fate of the Unfertilized Egg 83(i

G. Formation of the Second Polar Body, 837

H. Pronuclei Formation, Syngamy, and First Segmentation Division 838

I. Fate of the Cytoplasmic Components of the Fertilizing Sperm Flagellum. 841

J. Supernumerary Spermatozoa and Polyspermy in Mammalian Ova. . . 844

K. Stages of Development and Location of Eggs 845

L. The Age of the Egg at the Time of Fertilization 848

M. Implantation 850

N. Spacing and Orientation of Ova in Utero 852

O. Blastocyst Expansion 858

P. Embryo-endometrial Relationships . . 860

V. References 8(i5