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- 16:29, 7 August 2012 Z3374173 talk contribs uploaded File:Critical stages of the development of the primitive streak in the chick embryo..png (Wnt 8c expression during formation of Koller's sickle and the primitive streak. (A, B) At stage HH1 [39] Wnt8c (A) and Chordin (B) RNAs are expressed in the Area Opaca. At this time the embryo is growing and the epiblast increases in size as shown by the )