Mouse Tooth Development Movie

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Mouse Tooth Development

Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo (E 12.5–13.5). Co-expressing Fucci-G0/G1 probe and H2BEGFP. The tooth germ was cultured for 5 days ex vivo, and the images were taken at 30-min intervals. The lingual side is on the left.

Tooth morphogenetic dynamics viewed by an ex vivo time-lapse imaging system using transgenic mouse embryos co-expressing the Fucci-G0/G1 probe (Cdt1-mKO) and enhanced green fluorescent protein-labeled human histone 2B (H2B-EGFP).

  • Fluorescence ubiquitination cell cycle indicator - (FUCCI) whereby G0/G1 cells express a red fluorescent protein and S/G2/M cells express a green fluorescent protein.
  • Histone 2B - (H2B-EGFP) labels nucleus incorporated into nucleosomes without affecting cell cycle progression.

Note - Original research movie has been modified by the addition of labelling. Tooth stage labelling is only approximate.


Links: MP4 | Tooth Development | Mouse Development
bud cap bell
bud stage cap stage bell stage
E 12.5 E 14.5 E 15.5
Mouse-tooth-icon.jpg
 ‎‎Tooth E12.5-17
Page | Play

Original Research Movie

Links: MP4

Reference

Morita R, Kihira M, Nakatsu Y, Nomoto Y, Ogawa M, Ohashi K, Mizuno K, Tachikawa T, Ishimoto Y, Morishita Y & Tsuji T. (2016). Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations. PLoS ONE , 11, e0161336. PMID: 27588418 DOI.

Morita R, Kihira M, Nakatsu Y, Nomoto Y, Ogawa M, Ohashi K, et al. (2016) Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations. PLoS ONE 11(9): e0161336. https://doi.org/10.1371/journal.pone.0161336

Copyright

© 2016 Morita et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161336 S2 Video (MP4). https://doi.org/10.1371/journal.pone.0161336.s010


Cite this page: Hill, M.A. (2019, August 26) Embryology Mouse Tooth Development Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Tooth_Development_Movie

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© Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G