File talk:Age-related chromosome segregation errors during MI model.jpg
Nat Commun. 2015 Jul 1;6:7550. doi: 10.1038/ncomms8550.
Sakakibara Y1, Hashimoto S2, Nakaoka Y2, Kouznetsova A3, Höög C3, Kitajima TS1.
Abstract
The frequency of chromosome segregation errors during meiosis I (MI) in oocytes increases with age. The two-hit model suggests that errors are caused by the combination of a first hit that creates susceptible crossover configurations and a second hit comprising an age-related reduction in chromosome cohesion. This model predicts an age-related increase in univalents, but direct evidence of this phenomenon as a major cause of segregation errors has been lacking. Here, we provide the first live analysis of single chromosomes undergoing segregation errors during MI in the oocytes of naturally aged mice. Chromosome tracking reveals that 80% of the errors are preceded by bivalent separation into univalents. The set of the univalents is biased towards balanced and unbalanced predivision of sister chromatids during MI. Moreover, we find univalents predisposed to predivision in human oocytes. This study defines premature bivalent separation into univalents as the primary defect responsible for age-related aneuploidy.
PMID 26130582
http://www.nature.com/ncomms/2015/150701/ncomms8550/full/ncomms8550.html#supplementary-information
