File:Zygote replication z5039628.png

Picture description: DNA methylation and hydroxymethylation patterns during replication. Representative z-section images of maternal PN (left) and paternal PN (right) in 1-cell embryos with various replication statuses: from pre-replication to post-replication. A) Double immunostaining of 5MeC (upper row) and DIG dUTP (middle row). Merged images are shown on the lower row (green: 5MeC; red: replication); a total of 89 embryos were analyzed. B) Double immunostaining of 5hMeC (upper row) and BrdU (middle row). Merged images are shown on the lower row (green: 5MeC; red: replication); a total of 226 embryos were analyzed. Scale Bar: 5 µm.

Article title: 5-Methylcytosine and 5-Hydroxymethylcytosine Spatiotemporal Profiles in the Mouse Zygote

Authors: Juliette Salvaing, Tiphaine Aguirre-Lavin, Claire Boulesteix, Gaëtan Lehmann, Pascale Debey, and Nathalie Beaujean

Article link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364968/

Picture link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3364968/figure/pone-0038156-g002/

Article copyright statement: Copyright Salvaing et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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