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==Patterns of zona pellucida deposition and ZPC-ubiquitin colocalization in porcine ocyte-cumulus complexes isolated from small antral follicles.==
==Zona Pellucida and ZPC- ubiquitin==


(a, b) Accumulation of ZPC (gray) in ridges (arrowheads) adjacent to zona-adhering corona radiata cells (blue  =  nuclei stained with DAPI). (c-e) Colocalization of ZPC (red) and ubiquitin (green) in the zona pellucida (c, d) and in the cytoplasm of corona radiata cells (e); DNA was counterstained with DAPI (blue). Western blotting of ZPC protein in the isolated cumulus/corona cells from 60 oocyte cumulus complexes (lane 1), in 60 zona-free oocytes (lane 2) and in soluble zona proteins isolated from 60 oocytes (lane 3).
(a, b) Accumulation of ZPC (gray) in ridges (arrowheads) adjacent to zona-adhering corona radiata cells (blue  =  nuclei stained with DAPI). (c-e) Colocalization of ZPC (red) and ubiquitin (green) in the zona pellucida (c, d) and in the cytoplasm of corona radiata cells (e); DNA was counterstained with DAPI (blue). Western blotting of ZPC protein in the isolated cumulus/corona cells from 60 oocyte cumulus complexes (lane 1), in 60 zona-free oocytes (lane 2) and in soluble zona proteins isolated from 60 oocytes (lane 3).


(Original legend used)  
(Original figure legend, image based on date from PMID 21383844)


===References===
===References===


PMID 21383844
<pubmed>21383844</pubmed>| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044170/]
 


Original file name:
Pone.0017256.g006.jpg


===Copyright===  
===Copyright===  


Copyright Zimmerman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright Zimmerman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Original file name: Pone.0017256.g006.jpg


{{Template:Student Image}}
{{Template:Student Image}}

Revision as of 14:45, 14 August 2015

Zona Pellucida and ZPC- ubiquitin

(a, b) Accumulation of ZPC (gray) in ridges (arrowheads) adjacent to zona-adhering corona radiata cells (blue  =  nuclei stained with DAPI). (c-e) Colocalization of ZPC (red) and ubiquitin (green) in the zona pellucida (c, d) and in the cytoplasm of corona radiata cells (e); DNA was counterstained with DAPI (blue). Western blotting of ZPC protein in the isolated cumulus/corona cells from 60 oocyte cumulus complexes (lane 1), in 60 zona-free oocytes (lane 2) and in soluble zona proteins isolated from 60 oocytes (lane 3).

(Original figure legend, image based on date from PMID 21383844)

References

<pubmed>21383844</pubmed>| [1]


Copyright

Copyright Zimmerman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Original file name: Pone.0017256.g006.jpg


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