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==Patterns of zona pellucida deposition and ZPC-ubiquitin colocalization in porcine ocyte-cumulus complexes isolated from small antral follicles.==


(a, b) Accumulation of ZPC (gray) in ridges (arrowheads) adjacent to zona-adhering corona radiata cells (blue  =  nuclei stained with DAPI). (c-e) Colocalization of ZPC (red) and ubiquitin (green) in the zona pellucida (c, d) and in the cytoplasm of corona radiata cells (e); DNA was counterstained with DAPI (blue). Western blotting of ZPC protein in the isolated cumulus/corona cells from 60 oocyte cumulus complexes (lane 1), in 60 zona-free oocytes (lane 2) and in soluble zona proteins isolated from 60 oocytes (lane 3).
(Original legend used)
===References===
PMID 21383844
Original file name:
Pone.0017256.g006.jpg
Pone.0017256.g006.jpg


PMID 21383844
===Copyright===
 
Copyright Zimmerman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 
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Revision as of 14:39, 14 August 2015

Patterns of zona pellucida deposition and ZPC-ubiquitin colocalization in porcine ocyte-cumulus complexes isolated from small antral follicles.

(a, b) Accumulation of ZPC (gray) in ridges (arrowheads) adjacent to zona-adhering corona radiata cells (blue  =  nuclei stained with DAPI). (c-e) Colocalization of ZPC (red) and ubiquitin (green) in the zona pellucida (c, d) and in the cytoplasm of corona radiata cells (e); DNA was counterstained with DAPI (blue). Western blotting of ZPC protein in the isolated cumulus/corona cells from 60 oocyte cumulus complexes (lane 1), in 60 zona-free oocytes (lane 2) and in soluble zona proteins isolated from 60 oocytes (lane 3).

(Original legend used)

References

PMID 21383844

Original file name: Pone.0017256.g006.jpg

Copyright

Copyright Zimmerman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Note - This image was originally uploaded as part of an undergraduate science student project and may contain inaccuracies in either description or acknowledgements. Students have been advised in writing concerning the reuse of content and may accidentally have misunderstood the original terms of use. If image reuse on this non-commercial educational site infringes your existing copyright, please contact the site editor for immediate removal.

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