File:Z3333865.homologous recombination and c-MYC2 expression in ES cell clones.png
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Confirmation of homologous recombination and c-MYC2 expression in ES cell clones.
(A) Genomic DNA of ES cell clones 1, 14, 18 and 19 and of wildtype ES cells (wt Bruce 4) was digested with EcoRI. Digested DNA was analyzed by Southern blotting with a 5′ probe and a 3′ probe. (wt) DNA fragment of the wildtype c-Myc locus; (rec.) DNA-fragment of recombined hc-Myc locus. (B) Protein extracts were prepared of ES cell clones 1, 14, 18 and 19 as well as of wildtype ES cells (wt Bruce 4) and of a human lymphoblastoid cell line (LCL 1.11). Human c-MYC2 (hu. c-MYC, ca. 62 kDa) was detected with antibody clone Y69. In wildtype ES cells murine c-MYC2 (mu. c-MYC, ca. 64 kDa) was detected. For loading control an antibody specific for glyceraldehyde-3-phosphat-dehydrogenase (GAPDH; ca. 36 kDa) was used. Western blot results were reproduced five times.
Lehmann FM, Feicht S, Helm F, Maurberger A, Ladinig C, et al. (2012) Humanized c-Myc Mouse. PLoS ONE 7(7): e42021. doi:10.1371/journal.pone.0042021
Copyright: © 2012 Lehmann et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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| current | 12:04, 1 August 2012 | | 1,519 × 1,099 (282 KB) | Z3333865 (talk | contribs) | '''Confirmation of homologous recombination and c-MYC2 expression in ES cell clones.''' (A) Genomic DNA of ES cell clones 1, 14, 18 and 19 and of wildtype ES cells (wt Bruce 4) was digested with EcoRI. Digested DNA was analyzed by Southern blotting with |
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