File:Steps in a classic array CGH-analysis used for the most common chromosomal abnormalities.png

From Embryology

Original file(601 × 814 pixels, file size: 283 KB, MIME type: image/png)

This figure shows the steps in BAC array CGH. (1) BAC clones are selected from a physical map of the genome. (2) DNA samples are extracted from selected BAC clones and their identity is confirmed by DNA fingerprinting or sequence analysis. (3) A multi-step amplification process generates sufficient material from each clone for array spotting. Each clone is spotted in replicate onto a solid support. (4) Reference DNA and test DNA are differentially labeled with cyanine 3 and cyanine 5 respectively. (5) The two labeled products are combined and hybridized onto the spotted slide. (6) Images from hybridized slides are obtained by scanning in two channels. Signal intensity ratios from individual spots can be displayed as a simple plot (7) or by using more complex software such as SeeGH, which can display copy number alterations throughout the whole genome (8).

Copyright:This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.[1]

Reference

  1. Stefano G., Erika C., Francesca M., Giusy S., Francesca G., Michela B., Anna M. N., Antonio N., Ercole B., Laura B. and Giuseppe N.(2010) Design, Construction and Validation of Targeted BAC Array-Based CGH Test for Detecting the Most Commons Chromosomal Abnormalities. ‘’Genomic Insights.’’ 3:9-21


Assessment

+ genetic analysis technique is relavent to group topic. - Legend looks like it came directly from figure without any further explanation or interpretation for student learning criteria. - There is no link back to the original article and the included reference cannot be found on PubMed.



Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Students have been advised in writing concerning the reuse of content and may accidentally have misunderstood the original terms of use. If image reuse on this non-commercial educational site infringes your existing copyright, please contact the site editor for immediate removal.




Cite this page: Hill, M.A. (2024, June 17) Embryology Steps in a classic array CGH-analysis used for the most common chromosomal abnormalities.png. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Steps_in_a_classic_array_CGH-analysis_used_for_the_most_common_chromosomal_abnormalities.png

What Links Here?
© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G

File history

Yi efo/eka'e gwa ebo wo le nyangagi wuncin ye kamina wunga tinya nan

GwalagizhiNyangagiDimensionsUserComment
current00:16, 5 October 2011Thumbnail for version as of 00:16, 5 October 2011601 × 814 (283 KB)Z3289829 (talk | contribs)This figure shows the steps in BAC array CGH. (1) BAC clones are selected from a physical map of the genome. (2) DNA samples are extracted from selected BAC clones and their identity is confirmed by DNA fingerprinting or sequence analysis. (3) A multi-ste