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==Sperm binding test using unfertilised and fertilised human oocytes==

Unfertilised human oocytes recovered after IVF failure (A) and fertilised oocytes with a high fragmentation rate two days after fertilisation (B), were cleared of bound spermatozoa and re-inseminated. After three hours, oocytes were similarly washed by repeated aspiration in a pipette, and the number of spermatozoa bound to the surface was determined under a microscope. Original magnification ×400.

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===Reference===
<pubmed>17147816</pubmed>

Patrat, C., Auer, J., Fauque, P., Leandri, R. L., Jouannet, P., & Serres, C. (2006). Zona pellucida from fertilised human oocytes induces a voltage-dependent calcium influx and the acrosome reaction in spermatozoa, but cannot be penetrated by sperm. BMC Developmental Biology, 6, 59. http://doi.org/10.1186/1471-213X-6-59

===Copyright===

Copyright © 2006 Patrat et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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