File:Pone.0015329.g001 z5039628.jpg

Picture Description: Photographic examples representing the different scores of blastocysts donated for stem cell derivation.

HS475. Fully hatched blastocyst with clear tightly packed ICM. This embryo generated a new hESC line, HS475. Exp462. Hatching blastocyst with average number of cells in ICM, even trophectoderm layer. Exp472. Hatching blastocyst. Trophectoderm layer is uneven and the cells in the ICM are few in number and loosely packed. Exp404. Fully expanded blastocyst with clear tightly packed ICM and even trophectoderm layer. Exp439. Fully expanded blastocyst, with thin zona pellucida. Small ICM and trophectoderm cells of different size. Exp412. Fully developed blastocoel. Zona pellucida is still thick, meaning that the blastocyst is not fully expanded. Exp367. Early blastocyst. HS361. Early blastocyst with blastocoels filling less than 50% of embryo volume. This embryo generated the hESC line HS361. Exp457. This embryo is still compacted and has not reached blastocyst stage. All pictures were taken with a 40x objective, phase contrast or DIC, scale bar 50 µm. (Exp =  experiment number. HS is used as a prefix for established hESC lines at Karolinska Institutet).

Picture Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3013107/figure/pone-0015329-g001/

Article Title: No Relationship between Embryo Morphology and Successful Derivation of Human Embryonic Stem Cell Lines

Article Authors: Susanne Ström, Kenny Rodriguez-Wallberg, Frida Holm, Rosita Bergström, Linda Eklund, Anne-Marie Strömberg, and Outi Hovatta

Article Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3013107/

Published online 2010 Dec 31. doi: 10.1371/journal.pone.0015329

PMC copyright statement: Copyright Ström et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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