File:Nuclear accumulation of Hsp70 mRNA caused by Fragile X premutation rCGG repeats.png

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Nuclear accumulation of Hsp70 mRNA caused by Fragile X premutation rCGG repeats

Figure 3. Fragile X premutation rCGG repeats cause the nuclear accumulation of Hsp70 mRNA. A. Quantitative analysis of Hsp70 mRNA levels by real-time PCR from the adult heads of genotypes: +/+ (wild-type (WT)); elav; rCGG60 (rCGG-expressing homozygous flies); elav/+; rCGG60/+ (rCGG-heterozygous flies); Rm62LOF/+ (Rm62 mutation heterozygous flies); and rCGG60/+; Rm62LOF/+ (interaction). Housekeeping ribosomal protein 32 (Rpl32) mRNA was used as an internal control. *: p<0.05 B. Western blot with anti-histone 3 antibody, and α tubulin. C. Quantitative analysis of Hsp70 mRNA levels by real-time PCR on cytoplasmic and nuclear RNA fractions obtained from adult heads of wild-type (WT) and rCGG-expressing flies. Rpl32 mRNA was used as control. D. Quantitative analysis of Hsp70 mRNA levels in total RNA fractions upon heat shock. Both wild-type (WT) and rCGG-expressing flies were heat shocked for 30 min. No heat shock (NHS) represents non-heat shocked controls. Flies were decapitated at the indicated time after heat shock. Heads were collected and total RNA isolated from them. Both WT and rCGG-expressing flies displayed robust expression of Hsp70 in response to heat shock. After the removal of heat shock, Hsp70 transcripts declined radically in the WT, whereas in rCGG-expressing flies, Hsp70 transcripts show prolonged accumulation. Control samples do not display any overt differences in the timing or expression levels of Hsp70 during the initial response to a short heat shock. Real time against Fmr1 serves as a control on fractionated samples. The data represent mean ± SEM, n = 3.

Original file name: Journal.pgen.1002102.g003.png

Copyright: © 2011 Qurashi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Reference: <pubmed> 21655086</pubmed>| PMC3107199


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Cite this page: Hill, M.A. (2024, June 22) Embryology Nuclear accumulation of Hsp70 mRNA caused by Fragile X premutation rCGG repeats.png. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Nuclear_accumulation_of_Hsp70_mRNA_caused_by_Fragile_X_premutation_rCGG_repeats.png

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current11:32, 8 October 2011Thumbnail for version as of 11:32, 8 October 2011541 × 600 (163 KB)Z3290808 (talk | contribs)==Nuclear accumulation of Hsp70 mRNA caused by Fragile X premutation rCGG repeats== Figure 3. Fragile X premutation rCGG repeats cause the nuclear accumulation of Hsp70 mRNA. A. Quantitative analysis of Hsp70 mRNA levels by real-time PCR from the adult

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