Mice model and limb development

Description

Gross morphology and bone structure ofFgf-10 −/− mice. Gross photographs of E9.5 (A) and E17.5 (B)Fgf-10 −/− conceptuses are shown with Fgf-10 +/− andFgf-10+/+ littermates for comparison. (A, dorsal view) A complete absence of forelimb buds (arrow) in the Fgf-10−/− embryo relative to the presence of a forelimb bud (FB) in Fgf-10+/+ andFgf-10+/− littermates; (B, lateral view) complete absence of both limbs in theFgf-10−/− near-term fetus; (C–H) Gross photographs of E18.5 termFgf-10−/− (F–H) and wild-type (C–E) fetuses that have undergone skeletal double staining. Both wild-type (C–E) andFgf-10−/− (F–H) fetuses have scapulas with a blue-stained cartilaginous cap (S inC,D,F, and G) and pelvic girdles (arrowheads in E and H). However, theFgf-10−/− pelvic girdle is rudimentary and entirely cartilaginous (blue stained), whereas the wild-type pelvic girdle contains both bone (arrowhead directed at red-stained area) and cartilage (arrowhead directed at blue-stained area). The head of the humerus (blue-stained area labeled H) is visible only in the wild-type fetus (C,D) and obscures the scapula in the ventral view (D).

Copyright and References

<pubmed>15632068 </pubmed>
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