File:Gene morula.JPG

Gene_morula.JPG

Gene expression in dsWdr74 morula.

A–B. E-cadherin (Cdh1) localization by immunofluorescence marks blastomere cell-cell adhesion as expected in dsGFP morula (A). E-Cadherin is appropriately localized but present at reduced in dsWdr74 morula (B). C. qRT-PCR assays show reduced RNA polymerase II derived transcripts of Pouf51, Tead4, Actβ, GapdH, Bax, and Cdh1 but Trp53 shows an increase in transcripts in Wdr74-deficinet embryos. D. The average number of cells in dsGFP and dsWdr74 morula is not significantly different. E–F. Localization of Trp53 by immunofluorescence shows a marked increase of Trp53 protein in dsWdr74 embryos (compare F to E), consistent with the increase in Trp53 mRNA. Results of student T-test shown, error bars represent standard deviation. All data shown normalized to embryo equivalents. N.S., not significant. Scale bar in B and F representative for A–B and E–F, respectively.

Reference

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0022516

Citation: Maserati M, Walentuk M, Dai X, Holston O, Adams D, et al. (2011) Wdr74 Is Required for Blastocyst Formation in the Mouse. PLoS ONE 6(7): e22516. doi:10.1371/journal.pone.0022516

Copyright: © 2011 Maserati et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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