File:Effect-of-vegf-on-retinal-vasculature.JPG

Effects of astrocyte-derived VEGF on retinal vascular development

"Immunohistochemistry, visualizing endothelial cells (claudin 5, green in A, B, C, E, G) and retinal astrocytes (GFAP, red in A, B), shows that angiogenic sprouting at the leading edge of the growing vascular plexus appears normal in control (A) and mutant (B) animals lacking astrocyte derived VEGF. (C, D) Measurement of the surface area of the retinal vasculature (C) showed significantly reduced spreading in Vegfc/c mutants at P5 but not at P10 and not in P5 Hif1ac/c mutants at P5. (E) AT P10 proliferation (anti-BrdU in red) occurs predominantly in veins and is reduced in animals lacking astrocyte-derived VEGF in comparison to littermate controls. (G–H) Endothelial cell survival near arteries was assessed by measuring the width of the capillary free zone (CFS, white arrows) and the number of artery side branches (black arrowheads). (H) In mutant animals CFS width was not affected but the number of side branches was reduced. Scale bars are 50µm in A, 1000µm in C and 200µm in G; * is p<0.05 and ** is p<0.01."

doi:10.1371/journal.pone.0011863.g002

Reference

<pubmed>20686684</pubmed>

Copyright

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