File:Antibody immunostaining of ligands and receptors in triploid human embryos.png

Introduction of Specific Antibodies Generate Green Signals in Triploid Human Embryos under Immunofluorescence

Discarded zygotes obtained from IVF program used to form triploid embryos. Specific antibodies were then introduced against various ligand-receptor pairs such as IGF-1/IGF-1 receptor in which green signals were yielded due to immunofluorescence. Red signals were generated as propidium iodide was used to stain embryonic nuclei. Furthermore, growth factors were used to aid in embryonic development and promote blastocyst growth as well as IgG being applied to the negative control samples.

Reference

Kawamura K, Chen Y, Shu Y, Cheng Y, Qiao J, et al. (2012) Promotion of Human Early Embryonic Development and Blastocyst Outgrowth In Vitro Using Autocrine/Paracrine Growth Factors. PLoS ONE 7(11): e49328. doi:10.1371/journal.pone.0049328

Copyright

© 2012 Kawamura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0049328

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