Abnormal heart and caudal fin development in zebrafish due to Rap 1 knock down

Ubiquitous expressions of (A) rap1a and (B) rap1b were shown at 24 hpf. rap1MO injection led to the heart and caudal fin-fold phenotypes and curved trunk (arrows in C) at 48 hpf compared to wild type fish, and rap1 mRNA partially rescued the heart malformation in rap1 animals (C). The statistics indicates the proportion of fish embryos with heart phenotype was significantly decreased by rap1 mRNA rescue (D). mp157e fish was used to show the heart morphology of wild type, morphant and rescued morphant at 48 hpf at a higher magnification, the atrium (red), ventricle (green) and cardiocoelom (white) are marked by color dotted lines (E). In rap1MO larvae, the heart apparently failed to develop properly, while rap1 mRNAs partially restored the heart looping failure. Scale bar, 150 µm in A, B; 250 µm in C; 100 µm in E. Columns and error bars in D show mean±S.D. n>300 in each group.

--Mark Hill All required information is here. You might in future think about using a slightly larger image for uploading (up to 1000px wide) as it is quite difficult to see detail in this figure at the current size.

Reference

Reference

<pubmed>23226434</pubmed>

Copyright

© 2012 Dong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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