Difference between revisions of "2012 Group Project 3"

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*[http://www.ncbi.nlm.nih.gov/pubmed/8241557 Evidence for stimulus access to taste cells and nerves during development: an electron microscopic study.] <ref><pubmed>8241557</pubmed></ref>
*[http://www.ncbi.nlm.nih.gov/pubmed/8241557 Evidence for stimulus access to taste cells and nerves during development: an electron microscopic study.] <ref><pubmed>8241557</pubmed></ref>
<ref name="PMID8955790"/>
<ref name="PMID8955790"><pubmed>8955790</pubmed></ref>
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==History of Discoveries==
==History of Discoveries==

Revision as of 22:22, 18 September 2012

Taste Development

Introduction to the Gustatory System


Timeline of Developmental Processes of the Gustatory System

[5] Cite error: Closing </ref> missing for <ref> tag

Adult Tongue and Taste Buds – Structure and Function

The tongue is for tasting, swallowing, and speech.The tongue is located on the floor of the oral cavity, It is a muscular structure with sensory units crowning. The tongue is divided into an anterior two thirds and a posterior one third. These regions are divided by a V-shaped groove at the back of the tongue (sulcus terminalis). The anterior two thirds of the tongue is covered by stratified squamous epithelium, It contains a roughened surface and has projections called papillae that vary in shape and number. The most numerous papillae are the filiform papillae, which function to provide a surface that aids in holding food on the tongue during chewing. The larger, less numerous fungiform papillae are scattered among the filiform papillae where as the Circumvallate papilae form a wide V at the sulcus terminalis. There are no papillae or taste buds located on the posterior third of the tongue, having mucosal folds and the lingual tonsils instead.

The Image Below is a very simplistic Hand drawn Diagram of the surface of the Tongue showing the locations of the different papillae & and also the sulcus terminalis, This image indicates the tongue from an above veiw

The functional unit of the taste bud is a taste cells, there are between 50 and 100 taste cells in each taste bud these taste cells represent all 5 different tastes. Historically is was believed that different areas of the tongue were responsible for different taste sensations although this has since been disregarded.[1] The papillae contain taste buds which are connected to the oral cavity via a taste pore, the function of the taste bud is to transmit a chemical signal from the oral cavity to a taste cell, this chemical signal is the converted into an electrical impulse and delivered to the brain via nerve fibres for interpretation.

The Image Below is a very simplistic Hand drawn Diagram of a taste bud, in an extreme close up, cross section view of the the taste bud unit, not to scale

Taste bud 1.jpg

Taste Map


(try to include technologies to detect abnormalities during pregnancy)

Knocking out P2X Receptors

Gustatory abnormalities has not been widely researched and what has been researched has been through animal testing, most commonly we have found on mice.

In Huang, 2008 [PMID:21940456] this team used the release of the neurotransmitter, ATP (adenosine triphosphate) as a quantitative measurement of gustatory sensation and taste. This was done by using a comparison of wild type (WT) and double knockout (DKO) mice. P2X receptors, P2X2 and P2X3 were knocked out in the DKO mice. The premise of this article was that knocking out P2X receptors reduces transmitter secretion of ATP in taste buds, therefore they cannot taste.

It should be noted that the taste buds in DKO are functional, but are not stimulated by the administration of tastants.

The transmission of release of ATP is secretion through gap junction hemichannels (pannexin 1 gap junction).

When both P2X2 and P2X3 are knocked out, no taste is elicited. However they found that if either P2X2 OR P2X3 was knocked out there was a taste response. So the inference made from this is that if one of the two receptors from the P2X family was knocked out there still can have taste response. The WT mice showed significant stimulation by tastants whereas DKO had little to no stimulation of ATP release.

Insert image 4 from article here

FGF signalling and genes

Sprouty, or Srpy, genes have been related to regulating the development of circumvillate papillae (CVP). The CVP are large dome shaped papillae, which form a 'V' just in front of the terminal sulcus.

By knocking out Spry genes using mice which had Spry1 and Spry2 knocked out showed that the number of CVP doubled. However, when Fibroblast Growth Factor gene ("Fgf10") was absent, the number of CVP was significantly reduced, if not completely absent. The correlation between Spry1/2 and Fgf10 is that Spry1/2 antagonises "Fgf10" to limit the size of the CVP progenitor placode. Exclusive expression of Fgf10 in the mesenchyme is necessary for the formation of CVP.

"'Insert figure 1 from article"'

Taste buds develop from the mesenchyme but require local signalling to properly differentiate. Some signalling factors for proper development of taste buds besides FGF are: Sonic Hedgehog (SHH), Bone Morphogenetic Proteins (BMPs), Epidermal Growth Factor (EGF).

Additionally, this proves that the anterior and posterior developments of the tongue are derived from embryonic tissues, where the anterior tongue is derived fromt he ectoderm and the posterior tongue is derived from the endoderm ??? check article (& why is it necessary to know why different areas of the tongue are derived from different areas

Current Research

We have an evolving understanding of Embryonic Taste development, through the use of state-of-the-art technology and research techniques we are able to make brilliant discoveries that continue to connect the dots of this amazing natural process of human development. The majority of research in taste development is involving mice, these mammals show similar embryonic pathways to humans and research is performed in ethical and humane methods.

the below image shows histological stains of a mice tongue


In an animal study using mice by Suzuki Y, Ikeda K, Kawakami K.(2011) Firstly nominating "Six Genes" as a major component in gustatory development, stating that deficiencies in certain Six genes (specifically Six1 & Six4) leads to poor development. Their research also highlights evidence of cooperative relationships between Six genes for normal advance. Understanding the role of certain genes along with the intrinsic relationships they hold is crucial for the ability to identify possible causes and correction of any abnormalities [6] Another Animal Study involving mice explores a new idea of Neural crest contribution in taste development, specifically the development of papillae and taste buds. Liu HX, Komatsu Y, Mishina Y, Mistretta CM. (2012) suggest that Neural crest cells travel to the location of the tongue in early embryonic stages, gain epithelium phenotypes, multiply and then differentiate to eventually form taste papillae. Appreciating embryonic courses allows constant monitoring throughout expansion of the foetus.[7] In an animal study conducted by Rothova M, Thompson H, Lickert H, Tucker AS.(2012) exploring the historically debated issue of endoderm contribution to tongue development showed promising evidence that position of taste buds are patterned by the border of ectoderm and endoderm derivative epithelium. [8] Research by Ozdener H, Spielman AI, Rawson NE.(2012) developing a culture which allows taste cells to survive for up to 12 months, empowers researchers to study the processes of proliferation, differentiation and function. [9] Brain-derived neurotrophic factor (BDNF) was identified to promote gustatory neuron development, along with identifying the embryonic precursor populations for cranial ganglia in mice through the use of fate mapping in a study by Harlow, Yang, Williams, Barlow (2011)[10] The WNT gene family has a function of signally proteins for various reason such as development, In 2010 Liu, Staubach Grosse, Walton, Saims, Gumucio, Mistretta explored recent findings on the role of WNT's in tongue and papillae development, Concluding that WNT/β-catenin is essential for fungiform papillae differing to WNT5a which proved to be principle in tongue development. [11]

The below image shows and example of tongue abnormalities, this is called "double tongue" each side has independent movement [2] Abnormal of tongue.jpg

Useful links


Image gallery


  1. <pubmed>8955790</pubmed>
  2. <pubmed>9455607</pubmed>
  3. <pubmed>9541477</pubmed>
  4. <pubmed>8241557</pubmed>
  5. <pubmed>8955790</pubmed>
  6. <pubmed>21978088</pubmed>
  7. <pubmed>22659543</pubmed>
  8. <pubmed>ref>22581563</pubmed>
  9. <pubmed>ref>22643728</pubmed>
  10. <pubmed>ref>PMC2674259</pubmed>
  11. <pubmed>ref>PMC2768563</pubmed>


External Links

External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.

--Mark Hill 12:22, 15 August 2012 (EST) Please leave the content listed below the line at the bottom of your project page.

2012 Projects: Vision | Somatosensory | Taste | Olfaction | Abnormal Vision | Hearing