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Here is your page. Li M, Zhao H-C, Li R, Yu Y, Qiao J (2014) Chromosomal Aberrations in In-Vitro Matured Oocytes Influence Implantation and Ongoing Pregnancy Rates in a Mouse Model Undergoing Intracytoplasmic Sperm Injection. PLoS ONE 9(7): e103347. doi:10.1371/journal.pone.0103347

In-vitro fertilisation (IVF) and In-vitro maturation (IVM) are potential ways to alleviate issues with gonadotrophin stimulation. IVF has been accepted as the prefered Assisted Reproductive technique because of the higher success rate then IVM, which has 7 to 12% probablitly of plantation. Previous studies have concluded that the low success rate is related to the etiology of infertility rather then the technique itself. The selection of specific oocytes based on morphological features can greatly increase chance of implantation.

The study goal is to analyse spindle and chromosome and spindle configurations during the maturation process and resulting pre- and post-implantation embryos.

Experiment 1: used immature oocytes collected and matured in-vitro, with 3 cultures one at 18, 20 and 22 hours. alpha-tubulin and chromosome configurations were imaged by immunofluorescnce method. Experiment 2: used intracytoplasmic sperm injection to fertilise IVM oocytes, the pre-implanation phase was then compared to that of fertilised IVo oocytes. Expierement 3: Resulting embryos where introduced to a Pseudo-pregnant mouse, followed by dissection of fetuses on days 6.5 and 12.5.

Results; Spindle assembly in expiement 1 showed 18h had less that 50% originally, then rose to 52% in metaphase,the 20 and 22 hour grounds have significantly higher abnormalities (p<0.05) though had lower then a fresh control group. In expierment 2 1072 immature oocytes were collected and cultured with 924 becoming mature at MII stage, with 18h and 20h groups having reduced gene expression compared to 22h and control. Experiment 3 no significant difference in embryos on day 6.5, with 18h having slightly lower fetal rate then other groups. Day 12.5 implantation showed a significantly reduced implantation rate in 18h group in comparison to control and 22 h groups.

Shows greater chance of chromosome abnormalities in embryos from IVM timing and the relationship with post-implantation development in mouse models. Full term development results average number of pups 18h=3, 20h=4.5, 22h =4.4, IVO=5.88 Shi W, Xu B, Wu L-M, Jin R-T, Luan H-B, et al. (2014) Oocytes with a Dark Zona Pellucida Demonstrate Lower Fertilization, Implantation and Clinical Pregnancy Rates in IVF/ICSI Cycles. PLoS ONE 9(2): e89409. doi:10.1371/journal.pone.0089409