Lab 2 --Z3332885 10:38, 1 August 2012 (EST) Lab 3 --Z3332885 11:05, 8 August 2012 (EST) Lab 4 --Z3332885 12:10, 15 August 2012 (EST) Lab 5 --Z3332885 11:58, 22 August 2012 (EST) Lab 6 --Z3332885 10:13, 29 August 2012 (EST) Lab 7--Z3332885 11:36, 12 September 2012 (EST) Lab 8 --Z3332885 11:26, 19 September 2012 (EST) Lab 9 --Z3332885 10:11, 26 September 2012 (EST) Lab 10 --Z3332885 10:36, 3 October 2012 (EST) Lab 12 --Z3332885 10:39, 17 October 2012 (EST)
Lab attendance logged for 11 practicals. --Mark Hill 07:52, 18 October 2012 (EST)
Lab 1 Assessment
1)Identify the origin of In Vitro Fertilization and the 2010 nobel prize winner associated with this technique and add a correctly formatted link to the Nobel page
Research into controlling fertility has been going on for a while, and many experiments for IVF has been conducted since the 1960s. In 1973 the first IVF pregnancy was achieved, though baby did not survive till birth. In the 1978 the first IVF baby was born in india. The baby named "Durga" was the work of physican Kolkata using primitive methods and household refrigeration. In 1979 the world’s second baby conceived by IV was born.
In 2010, the Nobel Prize for physiology medicine was awarded to Robert G. Edwards for his contribution in the development of In Vitro Fertilization. 
(2)Identify and add a PubMed reference link to a recent paper on fertilisation and describe its key findings (1-2 paragraphs)
Many embryos are discarded from IVF labratories because they are considered to be poor quality. These embryos are, however, proposed to be good sources for human embryonic stem cells (heSC). This article studied whether heSC could be isolated from poor quality embryos, while also evaluating the efficiency of different isolation methods. Their experiments involved 166 embryos. The embryos were culture in a blastocyst medium for 2 days so that inner cell masses could develope. The inner masses from 32 embryo cells were then isolated, 17 using a mechanical method, the other 15 by immunosurgery. The isolated ICMs were subcultivated, and the rates of ICM colony formation was measured. Both methods had similar success rate (P>0.05). However, the cultures that did work had normal hESC differentiation ability, which indicates heSC could be derived from poor embryos. 
1.http://en.wikipedia.org/wiki/In_vitro_fertilisation 2.Liu W, Yin Y, Long X, Luo Y, Jiang Y, Zhang W, Du H, Li S, Zheng Y, Li Q, Chen X, Liao B, Xiao G, Wang W, Sun X. Derivation and characterization of human embryonic stem cell lines from poor quality embryos. J Genet Genomics. 2009 PMID: 19376483
Mark Hill - Q1 Please do not use wikipedia as your source for anything in my course. I want a scientific resource, textbook or the Nobel website. We will also discuss now to correctly cite PubMed references in a class tutorial. Q2 I specifically wanted a recent paper on fertilisation, not IVF or blastocyst development. 8/10
Lab 2 Assessment
Question 1. Upload an image from a journal source relating to fertilization or the first 2 weeks of development as demonstrated in the practical class.
Question 2: Identify a protein associated with the implantation process, including a brief description of the protein's role.
L-selectin is an adhesion molecule belonging to the selectin family of proteins. In the implantation process, L-selectin on the uterine epithelial cells interacts with sLex molecule on the trophoblast to mediate adhesion. This interaction has also been shown to induce the subsequent apoptosis of the uterine epithelial cells. 1
1. Liu S, Yang X, Liu Y, Wang X, Yan Q. sLeX/L-selectin mediates adhesion in vitro implantation model. Mol Cell Biochem. 2011 Apr;350(1-2):185-92. PMID: 21197561
Mark Hill - Q1 Image successfully uploaded with summary information, reference, copyright. You have left the "Student image" template out of the summary. Q2 This is a good representative protein for implantation. 9/10
Lab 3 Assessment
1.Identify the difference between "gestational age" and "post-fertilisation age" and explain why clinically "gestational age" is used in describing human development.
Gestational age- time between the last menstrual period and birth (measured in weeks). Post-fertilizational age- time elasped from fertilization to birth.
Clinically, the gestational age is used to describe human development. Gestational age can easily be determined by working out the number of weeks since last menstrual period, whereas the post-fertilization age is hard often hard to determine. Knowing this number can help determine the morbidity and mortality of the baby. 1
2.Identify using histological descriptions at least 3 different types of tissues formed from somites.
Sclerotome- Vertebrae and ribs 2
Dermatome- The dermis of the dorsal skin 3
Myotome- Skeletal muscles of the back 4
Mark Hill - Both answers are correct, it is more than just the dorsal dermis, it is all body dermis. Please do not use wikipedia as your source for anything in my course, I want a scientific resource or textbook. 8/10
Lab 4 Assessment
1. Identify the 2 invasive prenatal diagnostic techniques related to the placenta and 2 abnormalities that can be identified with these techniques.
Amniocentesis - amniotic fluid containing fetal tissue is sampled from the amnion using a needle. The DNA from the sample is then tested for genetic abnormalities such as Down syndrome (trisomy 21) or Patau syndrome (trisomy 13). 1
Chorionic Villus Sampling - chorionic villus (placental tissue) is sampled and tested for chromosomal abnormalities. This procedure usually takes place at 10–12 weeks' gestation. Down syndrome and Tay-Sachs disease can be detected using this technique. 2
2. Identify a paper that uses cord stem cells therapeutically and write a brief (2-3 paragraph) description of the paper's findings.
This study was conducted to better understand hematopoictic and endothlial precursor cells from umbilical cord blood, and their possible use as cell-based therapies for ischemic diseases.
On a growth factor depleted matrigel, ALDH(hi) cells significantly increased tube formation in Human umbilical vein endothelial cells. Transplantation of ALDH(hi) cell was also observed to significantly improve the recovery of perfusion in an ischemic limb (femoral artery ligation), corelation with an increase in murine vWF(+) blood vessel and CD31(+) capillary densities. UCB ALDH(hi) cells promote vascular regeneration and can therefore be use used to develope therapeutic strategies in treating ischemic diseases.
3. Putman DM, Liu KY, Broughton HC, Bell GI, Hess DA. Umbilical Cord Blood-Derived Aldehyde Dehydrogenase-Expressing Progenitor Cells Promote Recovery from Acute Ischemic Injury. Stem Cells:2012,16(8)PMID: 22899443
Mark Hill - Both answers are correct. Please do not use wikipedia as your source for anything in my course, I want a scientific resource or textbook. 8/10
Lab 7 Assessment
1.Provide a one sentence definition of a muscle satellite cell (b) In one paragraph, briefly discuss two examples of when satellite cells are activated ?
Myosatellite cells are mononuclear progenitor cells, found between the sarcolemma and the basement membrane of terminally differentiated muscle cells. These cells represent a population of stem cells, which are quiescent normally, but can proliferate to regenerate muscles in response to injury. Two examples of statellite cells being activated are:
Mechanical stress- strenuous exercise produce tears in the myofibers
Chronic diseases- causing hypertrophy of muscle cells.
When activated, the satellite cells enter the cell cyle to produce myoblasts that fuse/replace the damage cells.
2.In one brief paragraph, describe what happens to skeletal muscle fibre type and size when the innervating motor nerve sustains long term damage such as in spinal cord injury?
When the innervating motor nerve of skeletal muscle is damage, the muscle fiber will no longer be receiving nerve impulses to contract, leading to paralysis. The inactivity of the muscle in the long term will also cause atrophy. This is marked by the loss of type I (slow) fibres and the muscle become predominantly composed of fast glycolytic type II fibres.
Mark Hill - At last scientific resources/references. 10/10
Lab 8 Assessment
Overall the information presented is clear and concise. The development section is well researched and provides information of each individual part of the eye. The introduction is very brief and needs more on the development of the eye (as the project is on the development part. I feel that the anatomy should be under a separate heading. It also has two images that the serve essentially the same purpose. The historical section needs more on recent discoveries not just ancient ones. More work needs to be done on the layout of the images as is often hard to determine which image relates to which text. For the current research section, I think the group would benefit if they give a summary of the research as opposed to just listing the articles.
The introduction is very detailed, with descriptions of the varies components of taste. Although informative, there needs to be more about the development of the different aspects of taste. I feel that the type 2 receptor part doesn't belong here as this section is here to introduce the topic (maybe put it in a separate heading or in the neural pathway section?). There also needs to be a diagram if you are to include this as if is hard to follow. The balance between text and images is good, though some of the images are not labelled or properly referenced. Histories of discoveries section is very detailed and the table was very easy to read. The section the development of the taste is very informative and shows a of effort is placed into the of research of the topic (as it is often hard when the topic is not well understood). The section on the Structure and Function of the adult tongue gives the anatomy of the tongue, and should come before the part on neural pathways. The current research provided descriptions of the research and their goal and is done well.
The introduction, though brief, captured my attention and made me want to read more. It did exactly what is was supposed to do; provide an introduction to the topic, while keeping it interesting. Both the history and the development timeline sections are well researched and referenced. For the timeline part, there are large chunks of text describing processes but with no images to support them. This makes it confusing and hard to follow (especially when describing the development process). The division of the abnormalities and the inclusion of the pathophysiology is very thorough and is done very well. This part was very interesting to read. Current research is also well structured, and more importantly, relates to the abnormalities describe above. Overall this group has done an exceptional job.
Your introduction is short, but did state what you will be duscussing and the purpose of the webpage. However I think it would help by having more on what the abormalites are and how they affect people suffering from it. The section on normal eye development had sufficient information, though a few images would help. The section on Abnormal Development and and the choice of subsection is very well thought out and presented. The texts were easy to read and very detailed. More importantly, they were backed up current research which shows information is up to date. However, be careful not to have too much on the molecular pathways as can be hard to understand (may be break them up a bit and relate them to the concept discussed). More attention needs to be paid the formatting of the text and the placement of the images but otherwise, great job.
The introduction is concise and straight to the point. It gave an overview of the webpage and clearly indentified the purpose. The use of humor is welcoming though I think that image of the dog is over the top. Due to the great choice of subheadings, the development part is very easy to follow. More images to accompany the text would make it easier to understand would help break up some of the text. The current research section feels lacking. Referencing need to improve as some paragraphs have none.
Mark Hill - Reasonable, but brief, peer assessment feedback given here. 10/10
Lab 9 Assessment
1. Identify and write a brief description of the findings of a recent research paper on development of one of the endocrine organs covered in today's practical.
In mice, Gata4 and Gata6 genes are linked to to pancreas developement, and their elimination cause pancrease agenesis. The simutaneous deletion of both these genes disrupts the prolifereation of pancreatic progenitor cells, and cause defects in the branching morphogenesis. Interestingly, it was found that the loss of only one these genes leads to mild pancreatic defects, and these defects are often resolved postnatally.
2. Identify the embryonic layers and tissues that contribute to the developing teeth.
Layers and tissue that contribute to the developing teeth are:
Ectoderm: form Ameloblasts that deposit proteins which later mineralize to form the enamel.
The neural crest: Majority of the dental papilla of the tooth arise of the neural crest. Cells include odontoblasts, osteoblasts, and cementoblast.
Mesoderm: Dental papilla cells that from blood vessels in the pulp of the tooth.
Reference: "Development of the teeth." n.pag. embryology.ch. Web. 2 Oct 2012. <http://www.embryology.ch/anglais/sdigestive/gesicht05.html>. 
Mark Hill - Both answers correct. Gata4 and Gata6 paper was also used by another student? 10/10
Lab 10 Assessment
Identify a recent research article (using the pubmed tags to cite) on iPS cells and summarise in a few paragraphs the main findings of the paper.
This article looks at the use of Liposomal Magnetofection (LMF) as a method of producing "intergration free" induced pluripotent stem (iPS) cells and compares the features of the resultant cells to those derived using viral vector systems.
Morphologically, the LMF-iPS cells showed no distinct differences compared to that of normal embryonic stem cells. The expression endoderm genes (α-fetoprotein and α-amylase), mesoderm genes (β-enolase and renin), and ectoderm genes (Map2 and β-tubulin) was observed and found to be express as normal. To verify the in vitro differential potential of these cells, the researcher performed PCR and immunostaining for expression of cardiac troponin I (TnI) and expression of endothelial-specific receptor tyrosine kinase repectively. These markers were found to be present in 2 of the LMF-iPS cell lines out of 7 and that there were no observable pluripotency differences due to the lack of integration.
To further analyze differentiation potential in vivo, transgene-integrated 1LMF-h-iPS 1 cells and integration-free 1LMF-h-iPS 2 cells were injected into the femoral muscles of immunodeficient mice. Terotoma with three germ layers was found in form both studies.
Although the LMF method doesn't solve many of problems posed by iPS cells, the use of a non-integrating vector system eliminates insertional mutagenesis that is often associated with viral vectors.
Overall, it was found that LMF provides an effective and reproducible method of producing integration free iPS cells.
Mark Hill - 10/10
- "Development of the teeth." n.pag. embryology.ch. Web. 2 Oct 2012. <http://www.embryology.ch/anglais/sdigestive/gesicht05.html>.