Template talk:Yamada Shiota Lo

Shige Yamada

Cecilia Lo (cel36@pitt.edu)

Kohei Shiota

http://apps.devbio.pitt.edu/humanatlas

Dev Dyn. 2010 Jun;239(6):1585-95. Developmental atlas of the early first trimester human embryo. Yamada S, Samtani RR, Lee ES, Lockett E, Uwabe C, Shiota K, Anderson SA, Lo CW. Source Laboratory of Developmental Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA. Abstract Rapid advances in medical imaging are facilitating the clinical assessment of first-trimester human embryos at increasingly earlier stages. To obtain data on early human development, we used magnetic resonance (MR) imaging and episcopic fluorescence capture (EFIC) to acquire digital images of human embryos spanning the time of dynamic tissue remodeling and organogenesis (Carnegie stages 13 to 23). These imaging data sets are readily resectioned digitally in arbitrary planes, suitable for rapid high-resolution three-dimensional (3D) observation. Using these imaging datasets, a web-accessible digital Human Embryo Atlas (http://apps.devbio.pitt.edu/humanatlas/) was created containing serial 2D images of human embryos in three standard histological planes: sagittal, frontal, and transverse. In addition, annotations and 3D reconstructions were generated for visualizing different anatomical structures. Overall, this Human Embryo Atlas is a unique resource that provides morphologic data of human developmental anatomy that can accelerate basic research investigations into developmental mechanisms that underlie human congenital anomalies.

PMID 20503356

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401072

The MRI system used for this study is a 7T super-conducting magnet located at the NIH Mouse Imaging Facility (MIF). The pulse sequence consisted of rapid snapshot flash (SNAP) gradient echo sequence with TR = 30-35 msec, and TE = 3.3-3.8 msec. The total scan time was 13-40 hours with the acquired MR signals averaged 12-48 times to achieve image resolution with image matrix of 256x256x256 to 512x512x512. The final resolution of the images ranged from 30-150μm/pixel.

Movies and Images

File:MRI Stage 13 atrioventricular junction.mov