Template talk:Cahalan
From Embryology
Lymphocyte Migration Speeds
T cells - 10–12 μm/min in the follicle diffuse cortex, peak velocities up to 30 μm/min.
B cells - 6 μm/min in the follicle diffuse cortex, peak velocities up to 20 μm/min.
Both cortical T cells and follicular B cells move in random directions following "guide cells".
Lymphocyte Guide Cells
FDC - Follicular Dendritic Cells, may guide B cells in the follicle.
FRC - Fibroblastic Reticular Cells, may guide T cells in the follicle.
Lymph Node 2-Photon labelling
- Red - Alexa Fluor 633–conjugated WGA image channel
- sinus space both as a weakly stained area in the WGA image channel
- Green - T lymphocytes labeled with 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester.
- Green - B lymphocytes labelled with antibody to B220 (fixed tissue)
Original template
- Links: Immune System Development | Lymph Node Development | Quicktime | Flash
- Movie Source: Michael D. Cahalan, Professor and Chair, Department of Physiology and Biophysics, University of California, Irvine. Lab Two-photon imaging movies reproduced with permission of Prof. Cahalan. Movies show lymphocyte traffic through adult lymph nodes. Cells were isolated from spleens and lymph nodes of BALB/c mice.
- T Cell Quicktime: Transendothelial migration | T cell zone | Medullary sinus | Sinus endothelial barrier | Bi-directional traffic | cross the sinus endothelial barrier
- T Cell Flash: Transendothelial migration | T cell zone | Medullary sinus | Sinus endothelial barrier | Bi-directional traffic | Cross the sinus endothelial barrier
References: <pubmed>16273098</pubmed>| Immunology <pubmed>18173372</pubmed>