The Effect of CSF-1R Expression on Developing Brain Architecture

The receptor for Colony Stimulating Factor 1 (CSF-1R) was shown by Erblich et al.^[1] to impact the developing brain architecture of embryonic mice through its effect on microglia numbers. Mice not expressing CSF-1R (Csf1r-/-) were found to have abnormal architecture and depleted microglia numbers.

A. Gross architecture of paraformaldehyde fixed brains from 3-week-old mice cut 0.5 mm anterior to the optic chiasm from three WT (1–3) and three Csf1r−/− (4–6) mice. Note the enlarged ventricles and thinned cortex in Csf1r−/− mice.

B. 5 µm Coronal sections stained with Nissl stain. 1, 2, 3, 4, anterior olfactory nucleus. 5, 6, Cortex, thalamus and hippocampus. 7, 8, cerebellum. 1, 3, 5, 7, WT, 2, 4, 6, 8, Csf1r−/−. Scale bar 1 mm.

C. 5 µm Coronal sections stained with Nissl stain of brains from WT and Csf1r−/− E16 mice. Bar = 500 µm.

D. 5 µm Coronal section through the cortex, thalamus and hippocampus from day 1PP WT (1) and Csf1r−/− (2) mice. Scale bar = 1 mm.

E. Close up of sections of the cortex from B5 and 6 with lines with double arrows indicating matched regions in which cell counts and measurements were made from WT and Csf1r−/−. Bar = 200 µm.

F. Cortical thickness from regions indicated in E. N = 3 for each genotype. p = 0.0168 Student's t test.

Reference

Copyright: © 2011 Erblich et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Original file name: Figure 2. doi:10.1371/journal.pone.0026317.g002 http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026317?imageURI=info:doi/10.1371/journal.pone.0026317.g002

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